Comparison of a radioactive and non-radioactive method for sequencing foot and mouth disease virus isolates
| Autor: | D V Rai, W C Carpenter, M C Höfner, A R Samuel |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Silver Staining
General Medicine Sequence Analysis DNA Biology biology.organism_classification Virology Polymerase Chain Reaction Silver stain Aphthovirus Isotope Labeling DNA Viral Animals Autoradiography Animal Science and Zoology Reagent Kits Diagnostic Dna viral Foot-and-mouth disease virus DNA Primers |
| Zdroj: | Revue scientifique et technique (International Office of Epizootics). 15(3) |
| ISSN: | 0253-1933 |
| Popis: | The authors compare the radioactive method of detecting foot and mouth disease virus sequence products with a non-radioactive, silver stain sequencing method. The latter was found to compare favourably to the radioactive technique for detecting such products. The silver stain sequencing method was simple and did not require expensive specialised equipment. This new approach will be particularly useful in developing countries, since the method does not depend on the availability of fresh radioactive isotopes and is also safer and considerably cheaper. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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