GENE SIGNATURES ASSOCIATED WITH ADAPTIVE HUMORAL IMMUNITY FOLLOWING SEASONAL INFLUENZA A/H1N1 VACCINATION

Autor: Diane E. Grill, Michael T. Zimmermann, Ann L. Oberg, Richard B. Kennedy, Hannah M. Salk, Inna G. Ovsyannikova, G.A. Poland, Iana H. Haralambieva
Jazyk: angličtina
Rok vydání: 2016
Předmět:
0301 basic medicine
Male
Gene Expression
Adaptive Immunity
medicine.disease_cause
Antibodies
Viral
Cohort Studies
0302 clinical medicine
Virus Neutralization Antibody Titer
Influenza A Virus
H1N1 Subtype
IL12A
Influenza A virus
Genetics (clinical)
RNA Sequencing
Hemagglutination-Inhibition Antibody Titer
Vaccination
virus diseases
Middle Aged
3. Good health
Titer
Influenza Vaccines
030220 oncology & carcinogenesis
Female
Seasons
Antibody
Influenza Vaccine
Human
animal structures
Influenza vaccine
Immunology
Biology
Article
03 medical and health sciences
Influenza A/H1N1 Virus
Mannosidases
Genetics
medicine
Humans
Aged
Hemagglutination assay
Geneset
Hemagglutination Inhibition Tests
Virology
Antibodies
Neutralizing
Immunity
Humoral
030104 developmental biology
Humoral immunity
biology.protein
Biomarkers
Zdroj: Genes and immunity
ISSN: 1476-5470
1466-4879
2010-2011
Popis: This study aimed to identify gene expression markers shared between both influenza hemagglutination inhibition (HAI) and virus-neutralization antibody (VNA) responses. We enrolled 158 older subjects who received the 2010-2011 trivalent inactivated influenza vaccine. Influenza-specific HAI and VNA titers and mRNA-sequencing were performed using blood samples obtained at Days 0, 3 and 28 post vaccination. For antibody response at Day 28 versus Day 0, several gene sets were identified as significant in predictive models for HAI (n=7) and VNA (n=35) responses. Five gene sets (comprising the genes MAZ, TTF, GSTM, RABGGTA, SMS, CA, IFNG and DOPEY) were in common for both HAI and VNA. For response at Day 28 versus Day 3, many gene sets were identified in predictive models for HAI (n=13) and VNA (n=41). Ten gene sets (comprising biologically related genes, such as MAN1B1, POLL, CEBPG, FOXP3, IL12A, TLR3, TLR7 and others) were shared between HAI and VNA. These identified gene sets demonstrated a high degree of network interactions and likelihood for functional relationships. Influenza-specific HAI and VNA responses demonstrated a remarkable degree of similarity. Although unique gene set signatures were identified for each humoral outcome, several gene sets were determined to be in common with both HAI and VNA response to influenza vaccine.
Databáze: OpenAIRE
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