Immunohistochemical Progesterone Receptor Assay: Measurement by Image Analysis
| Autor: | Irene J. Check, Cynthia Cohen, Nafissa El-Badawy, Patricia B. DeRose, Demetrios S. Sgoutas |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Pathology
medicine.medical_specialty medicine.drug_class Mammary gland Nuclear area Breast Neoplasms Cell analysis Biology Monoclonal antibody Biochemistry Sensitivity and Specificity Predictive Value of Tests Progesterone receptor Image Processing Computer-Assisted medicine Humans skin and connective tissue diseases Frozen section procedure Dextrans General Medicine Immunohistochemistry medicine.anatomical_structure Charcoal Progesterone receptor assay measurement Receptors Progesterone hormones hormone substitutes and hormone antagonists |
| Zdroj: | American Journal of Clinical Pathology. 96:704-710 |
| ISSN: | 1943-7722 0002-9173 |
| DOI: | 10.1093/ajcp/96.6.704 |
| Popis: | To determine the efficiency of image analysis in immunohistochemical progesterone receptor (PgR) measurement, 94 primary breast carcinoma tissue samples were evaluated for PgR by biochemical dextran-coated charcoal assay (DCC) and an immunohistochemical method. Frozen sections immunostained for PgR with a monoclonal antibody (Abbott PgR-ICA, Chicago, IL) and the peroxidase-antiperoxidase technique were scored semiquantitatively histologic score by microscopy and quantitatively (percentage nuclear area immunopositivity [PNA] using the CAS 200 image analyzer (Cell Analysis Systems, Elmhurst, IL). There was a positive correlation between dextran-coated charcoal assay and both histologic score (r = 0.82) and PNA (r = 0.69). Selected cutoff points of 60 histologic score and 6.5% PNA based on sensitivity/specificity calculations yielded a predictive value of a negative test of 73% and 80%, respectively, and a positive predictive value of 100% for both; ranges of fmol/mg protein PgR correspond to ranges of histologic score and PNA. The use of an image analyzer to measure PNA in PgR-immunostained sections is a viable alternative to dextran-coated charcoal assay, especially when insufficient fresh tissue is available. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|