Antimicrobial photodynamic therapy with photosensitizer in ethanol improves oxidative status and gingival collagen in a short-term in periodontitis

Autor: Fernanda Maia Pillusky, Roberto Marinho Maciel, Luciana Taschetto Vey, Karla Zanini Kantorski, Cristiane Cademartori Danesi, Luisa Machado Barin, Victor De Mello Palma, Marilise Escobar Burger, Raquel Cristine Silva Barcelos
Rok vydání: 2017
Předmět:
Zdroj: Photodiagnosis and Photodynamic Therapy. 19:119-127
ISSN: 1572-1000
DOI: 10.1016/j.pdpdt.2017.05.010
Popis: Background This study evaluated the antimicrobial photodynamic therapy (aPDT) effects using the methylene blue (MB) in ethanol 20% on systemic oxidative status and collagen content from gingiva of rats with periodontitis. Methods Rats were divided into five experimental groups: NC (negative control; no periodontitis); PC (positive control; periodontitis without any treatment); SRP (periodontitis and scaling and root planing), aPDT I (periodontitis and SRP + aPDT + MB solubilized in water), and aPDT II (periodontitis and SRP + aPDT + MB solubilized in ethanol 20%). After 7 days of removal of the ligature, the periodontal treatments were performed. At 7/15/30 days, gingival tissue was removed for morphometric analysis. The erythrocytes were used to evaluate systemic oxidative status. Results PC group showed higher lipoperoxidation levels at 7/15/30 days. aPDT indicated a protective influence in erythrocytes at 15 days observed by the elevation in levels of systemic antioxidant defense. aPDT II group was the only one that restored the total collagen area in 15 days, and recovered the type I collagen area at the same time point. Conclusions aPDT as an adjunct to the SRP can induce the systemic protective response against oxidative stress periodontitis-induced and recover the gingival collagen, thus promoting the healing periodontal, particularly when the MB is dissolved in ethanol 20%.
Databáze: OpenAIRE
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