| Autor: |
Du, Xiaoyue, Wen, Shaodi, Shi, Run, Xia, Jingwei, Wang, Ruotong, Zhang, Yihan, Pan, Banzhou, Wu, Xiaoliu, Zhu, Wei, Feng, Jifeng, Wang, Xin, Shen, Bo |
| Rok vydání: |
2023 |
| DOI: |
10.6084/m9.figshare.22616218 |
| Popis: |
Additional file 1: Supplement Fig. 1. Process of research and experimental gating strategy. (A) Diagram of peripheral blood sample collection, treatment and treatment efficacy evaluation. (B) Flowchart of study design. (C) Flow cytometry gating strategy. Leukocytes were first identified based on CD45 expression. Lymphocytes were identified based on forward (size) and side (granularity) scatted characteristics. Expression of CD3 identified Total T cells (CD3+), and were further subdivided based on CD4 and CD8 expression into TH(CD3+CD4+) and CTL(CD3+CD8+) cells. Expression of HLA-DR and CD56 identified the Activated T cells (HLADR+) and NKT cells (CD56+). Expression of CD19 / CD56 but do not express CD3 were identified as B cells (CD3-CD19+) and NK cells (CD3-CD56+). Supplement Fig. 2. Differences between DCB and NDB groups at baseline and post ICB treatment in the training. Nonparametric Mann-Whitney test was used for comparisons (using continuous variables). Durable clinical benefit; NDB, None durable benefit; NK, natural killer; NKT, natural killer T; CEA, carcinoembryonic antigen; CA125, carbohydrate antigen 125; CA199, carbohydrate antigen 199; NSE, neuron-specific enolase. Supplement Fig. 3. Overall population Kaplan–Meier curves for PFS(A) and OS(B). DCB and NDB group Kaplan–Meier curves for (C)PFS and (D)OS. P Values were calculated by log-rank statistics. Durable clinical benefit; NDB, None durable benefit; PFS, progression-free survival; OS, overall survival. Supplement Fig. 4. Kaplan–Meier curves forPFS. P Values were calculated by log-rank statistics. Supplement Fig. 5. Kaplan–Meier curves for OS. P Values were calculated by log-rank statistics. Supplement Fig. 6. Relationship between infiltrating lymphocytes and prognosis in NSCLC patients with TCGA. Whole-transcriptome RNA-seq data for 337 NSCLC cases and their corresponding clinical data were downloaded from the TCGA database ( https://portal.gdc.cancer.gov/ ). Immuno-infiltrating cells were calculated with the xCell R package. Patients’ parameters were categorized by optimal cut-off values (Low / High group), The survival curve was plotted using the Kaplan–Meier method and the log rank test was used to determine statistical significance; p |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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