Lymphatic absorption of α-linolenic acid in rats fed flaxseed oil-based emulsion
| Autor: | Laurence Fonseca, Nicole Combe, Sandrine Djoukitch, Leslie Couëdelo, Carole Boué-Vaysse, Emeline Montesinos, Maud Cansell |
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| Rok vydání: | 2010 |
| Předmět: |
Male
Linseed Oil food.ingredient Biological Availability Medicine (miscellaneous) Lecithin Intestinal absorption Bile Acids and Salts Lymphatic System food Essential fatty acid Linseed oil Flax Animals Rats Wistar Lipase Triglycerides chemistry.chemical_classification Nutrition and Dietetics Chromatography biology Intestinal lipid absorption Temperature alpha-Linolenic Acid Hydrogen-Ion Concentration Dietary Fats Rats Bioavailability Solubility chemistry Area Under Curve Emulsion biology.protein Emulsions |
| Zdroj: | British Journal of Nutrition. 105:1026-1035 |
| ISSN: | 1475-2662 0007-1145 |
| DOI: | 10.1017/s000711451000454x |
| Popis: | The bioavailability of α-linolenic acid (ALA) from flaxseed oil in an emulsified formv.a non-emulsified form was investigated by using two complementary approaches: the first one dealt with the characterisation of the flaxseed oil emulsion inin vitrogastrointestinal-like conditions; the second one compared the intestinal absorption of ALA in rats fed the two forms of the oil. Thein vitrostudy on emulsified flaxseed oil showed that decreasing the pH from 7·3 to 1·5 at the physiological temperature (37°C) induced instantaneous oil globule coalescence. Some phase separation was observed under acidic conditions that vanished after further neutralisation. The lecithin used to stabilise the emulsions inhibited TAG hydrolysis by pancreatic lipase. In contrast, lipid solubilisation by bile salts (after lipase and phospholipase hydrolysis) was favoured by preliminary oil emulsification. Thein vivoabsorption of ALA in thoracic lymph duct-cannulated rats fed flaxseed oil, emulsified or non-emulsified, was quantified. Oil emulsification significantly favoured the rate and extent of ALA recovery as measured by the maximum ALA concentration in the lymph (Cmax = 14 mg/ml at 3 h in the emulsion groupv.9 mg/ml at 5 h in the oil group;P v.26 mg × h/ml for rats fed oil;P in vitrostudies helped to partly interpret the physiological results. |
| Databáze: | OpenAIRE |
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