Identification of Papillomaviral DNA Sequences in Hairless Mouse Tumours Induced by Ultraviolet Irradiation
| Autor: | Vivienne E. Reeve, Lutz Gissmann, Paul J. Canfield, Clifford H. Gallagher, Gavin E. Greenoak, Jerzy K. Kulski, Peta A. Tilbrook |
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| Rok vydání: | 1989 |
| Předmět: |
Skin Neoplasms
Ultraviolet Rays Biopsy Cell Biology Mice Nucleic acid thermodynamics chemistry.chemical_compound Virology medicine Animals Papillomaviridae Mice Hairless Papilloma Epithelioma Hybridization probe Nucleic Acid Hybridization medicine.disease biology.organism_classification Hairless Disease Models Animal medicine.anatomical_structure chemistry Mastomys DNA Viral Carcinoma Squamous Cell DNA Probes Carcinoma in Situ DNA |
| Zdroj: | Journal of General Virology. 70:1005-1009 |
| ISSN: | 1465-2099 0022-1317 |
| Popis: | Papillomas, carcinomas in situ and squamous cell carcinomas were induced using ultraviolet irradiation in the hairless mouse strain Mus musculus HRA/Skh. DNA extracted from biopsies was examined using Mastomys natalensis papillomaviral DNA as a hybridization probe at reduced stringency. Sequences homologous to the probe were detected in 16 of 24 papillomas, five of five carcinomas in situ and six of 38 squamous cell carcinomas. A number of tumour DNAs (16/33) also hybridized with mixed DNAs of human papillomavirus types 11, 13, 16 and 18 at reduced stringency. This suggests a role for the hairless mouse as a laboratory model for the study of the involvement of papillomaviruses in malignant transformations. |
| Databáze: | OpenAIRE |
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