MicroRNA-152 inhibits tumor cell growth by directly targeting RTKN in hepatocellular carcinoma
| Autor: | Zhang Yanjun, Qiuju Shao, Liang Jun, Zhou Jiejing, Qi Yuhong, Yu Dequan |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Oncology Cancer Research medicine.medical_specialty Carcinoma Hepatocellular Colorectal cancer Apoptosis 03 medical and health sciences 0302 clinical medicine GTP-Binding Proteins Internal medicine Cell Line Tumor microRNA medicine Carcinoma Humans Cell Proliferation Oncogene business.industry Liver Neoplasms Intracellular Signaling Peptides and Proteins NF-kappa B Cancer General Medicine Cell Cycle Checkpoints Cell cycle medicine.disease digestive system diseases Gene Expression Regulation Neoplastic MicroRNAs 030104 developmental biology 030220 oncology & carcinogenesis Hepatocellular carcinoma Cancer research business Liver cancer Apoptosis Regulatory Proteins |
| Zdroj: | Oncology reports. 37(2) |
| ISSN: | 1791-2431 |
| Popis: | Hepatocellular carcinoma (HCC) is the most common form of adult liver cancer and accounts for approximately 90% of all cases of primary liver cancer annually. Rhotekin (RTKN), which functions as a cancer promoter, can be frequently detected in many human cancers, including gastric cancer, colorectal carcinoma and bladder carcinoma. The aim of this study was to investigate the role of RTKN in HCC. Using HCC cells and tissues from patients with liver cancer, we demonstrated that RTKN was significantly increased in HCC. To examine the effect of RTKN on HCC, RTKN overexpressed or silenced HepG2 and Hep3B cells were constructed. Cell proliferation and apoptosis were measured by RT-PCR and flow cytometry. The results showed that RTKN can function as an oncogene and promote the proliferation, while inhibiting apoptosis, of HepG2 and Hep3B cells. Furthermore, we identified that RTKN is a direct gene target of miR-152. miR-152 can reverse the growth promoting effect of RTKN on HCC cells through G2/M phase arrest and nuclear factor-κB (NF-κB) signal inhibition. In conclusion, our research identified that miRNA-152 can inhibit tumor cell growth by targeting RTKN in HCC. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|