Baicalin Suppresses the Proliferation and Migration of Ox-LDL-VSMCs in Atherosclerosis through Upregulating miR-126-5p
| Autor: | Gaoliang Yan, Zhongpu Chen, Long Chen, Genshan Ma, Xiaodong Pan, Zulong Sheng |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Male Vascular smooth muscle Flavonoid Myocytes Smooth Muscle Pharmaceutical Science Down-Regulation Pharmacology Chronic inflammatory disease HMGB1 Muscle Smooth Vascular 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Downregulation and upregulation Cell Movement Humans HMGB1 Protein Cell Proliferation chemistry.chemical_classification Flavonoids biology General Medicine Middle Aged Atherosclerosis Peripheral blood Up-Regulation Lipoproteins LDL MicroRNAs 030104 developmental biology chemistry 030220 oncology & carcinogenesis biology.protein Female Baicalin Lipoprotein |
| Zdroj: | Biologicalpharmaceutical bulletin. 42(9) |
| ISSN: | 1347-5215 |
| Popis: | Atherosclerosis (AS) is a chronic inflammatory disease threatening human health, and vascular smooth muscle cells (VSMCs) are involved in AS processes. Baicalin is a flavonoid compound, which has anti-atherosclerotic effect. The aim of our study was to explore the molecular mechanism of baicalin on AS. The expression of miR-126-5p was measured in peripheral blood of AS patients and healthy control. We found miR-126-5p expression was decreased in AS. Then, high-mobility group box 1 (HMGB1) was verified as a target of miR-126-5p and its expression was increased in AS. Similarly, miR-126-5p and HMGB1 expression was downregulated and upregulated in oxidized low-density lipoprotein treated VSMCs (ox-LDL-VSMCs), respectively. Furthermore, baicalin upregulated miR-126-5p and downregulated HMGB1 expression. Functionally, baicalin significantly inhibited ox-LDL-VSMCs proliferation and migration, and miR-126-5p targets HMGB1 to enhance the inhibition induced by baicalin. Taken together, baicalin is able to prevent AS, which suppressed the proliferation and migration of ox-LDL-VSMCs through upregulating miR-126-5p by targeting HMGB1. These findings suggested that baicalin is an effective drug to alleviate AS, and miR-126-5p is a novel therapeutic target for AS. |
| Databáze: | OpenAIRE |
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