The effect of heparin on fibronectin and thrombospondin synthesis by human smooth muscle cells
| Autor: | Nicholas A. Kefalides, Helen Conaway, Bernadette Lyons-Giordano |
|---|---|
| Rok vydání: | 1987 |
| Předmět: |
Vascular smooth muscle
Immunoprecipitation Cell Biophysics Enzyme-Linked Immunosorbent Assay In Vitro Techniques Biochemistry Muscle Smooth Vascular chemistry.chemical_compound Thrombospondin 1 medicine Humans Molecular Biology Cells Cultured Glycoproteins HEPES Thrombospondin biology Heparin Cell Biology Molecular biology Fibronectins Molecular Weight Fibronectin medicine.anatomical_structure chemistry biology.protein Thrombospondins Cell Division medicine.drug |
| Zdroj: | Biochemical and Biophysical Research Communications. 148:1264-1269 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/s0006-291x(87)80269-3 |
| Popis: | Summary Heparin causes increased synthesis of fibronectin and thrombospondin by human vascular smooth muscle cells as assessed by immunoprecipitation and ELISA techniques. More fibronectin and thrombospondin were immunoprecipitated from the medium of cells treated with 180 μ g/ml heparin than from that of control cells. Heparin did not effect levels of fibronectin and thrombospondin immunoprecipitated from the cell-matrix fractions. By ELISA, heparin was found to cause a 1.7 fold increase in medium fibronectin levels/cell and a 10 fold increase in medium thrombospondin levels/cell. Concomitantly, smooth muscle cells treated with 180 g/ml heparin for 48 h exhibited 55% decrease in proliferation relative to controls. |
| Databáze: | OpenAIRE |
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