Xenotransplantation of goat ovary as an alternative to analyse follicles after vitrification
| Autor: | Johan Smitz, Nathalie Jiatsa Donfack, C.H. Lobo, R.M.P. Rocha, Ana Paula Ribeiro Rodrigues, Marilia de Oliveira Taumaturgo, José Ricardo de Figueiredo, Ramon da Silva Raposo, Benner Geraldo Alves, Marcelo Bertolini, Jamily Bezerra Bruno, K.A. Alves, Regiane Rodrigues Dos Santos |
|---|---|
| Přispěvatelé: | Pathology/molecular and cellular medicine, Clinical Biology, Follicle Biology |
| Rok vydání: | 2018 |
| Předmět: |
Ovarian Cortex
Xenotransplantation medicine.medical_treatment Transplantation Heterologous Gene Expression Mice Nude Apoptosis Ovary ovarian function restoration Biology ovarian cryopreservation Cryopreservation Tissue Culture Techniques Andrology Mice 03 medical and health sciences 0302 clinical medicine Endocrinology Ovarian Follicle folliculogenesis Fibrosis Follicular phase medicine Animals RNA Messenger Mice Inbred BALB C 030219 obstetrics & reproductive medicine Goats 0402 animal and dairy science 04 agricultural and veterinary sciences medicine.disease Vitrification 040201 dairy & animal science Transplantation surgical procedures operative medicine.anatomical_structure graft Female Animal Science and Zoology Folliculogenesis Biotechnology |
| Zdroj: | Reproduction in Domestic Animals. 54:216-224 |
| ISSN: | 0936-6768 |
| Popis: | The aim of this study was to evaluate the caprine preantral follicles enclosed on vitrified/warmed ovarian cortex grafted to nude BALB/mice during 1 month. The ovarian cortex from goats was fragmented (3 × 3 × 0.5 mm) and divided into four groups: fresh control, vitrified control, fresh transplant and vitrified transplant. Follicular morphology, development and density, fibrosis as well as apoptosis, and tissue revascularization were evaluated. It was also observed a significant decrease in morphologically normal preantral (primordial, transition, primary and secondary) follicles in both vitrified control and vitrified transplant treatments when compared with both fresh control and fresh transplant. However, fresh control and fresh transplant exhibited a similar percentage of developing follicles. Additionally, Vitrified control showed a significant increase in developing follicles in comparison with both fresh control and fresh transplant. Follicular density significantly decreased in all treatments in comparison with fresh control. We observed high fibrosis in both fresh transplant and vitrified transplant. The mRNA expression of caspase 3 was lower in both fresh transplant and vitrified transplant in comparison with vitrified control. In conclusion, xenotransplantation is an excellent strategy to maintain normal preantral follicle morphology after vitrification/warming of goat ovarian tissue. Yet, in order to ensure the survival and development of these follicles, it is essential to improve the revascularization of the graft. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text