Altered integrin expression patterns shown by microarray in human cutaneous melanoma
| Autor: | Róza Ádány, Szilvia Ecsedi, Tímea Kiss, Laura Vízkeleti, Viktória Koroknai, Margit Balázs, István Szász, Orsolya Papp |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Adult
Male 0301 basic medicine Integrins Cancer Research Pathology medicine.medical_specialty Skin Neoplasms Microarray Integrin Dermatology Metastasis 03 medical and health sciences 0302 clinical medicine Downregulation and upregulation Biomarkers Tumor Tumor Cells Cultured medicine Humans Child Melanoma Aged Cell Proliferation Aged 80 and over biology Cell growth Gene Expression Profiling Middle Aged Prognosis medicine.disease Gene expression profiling 030104 developmental biology Oncology Lymphatic Metastasis 030220 oncology & carcinogenesis Cutaneous melanoma Disease Progression biology.protein Female Follow-Up Studies |
| Zdroj: | Melanoma Research. 27:180-188 |
| ISSN: | 0960-8931 |
| DOI: | 10.1097/cmr.0000000000000322 |
| Popis: | A large variety of molecular pathways in melanoma progression suggests that no individual molecular alteration is crucial in itself. Our aim was to define the molecular alterations underlying metastasis formation. Gene expression profiling was performed using microarray and qRT-PCR to define alterations between matched primary and metastatic melanoma cell lines. These data were integrated with publicly available unmatched tissue data. The invasiveness of cell lines was determined by Matrigel invasion assays and invasive clones from primary melanoma-derived cell lines were also selected. Two metastatic cell line models were created: the regional lymph node WM983A-WM983A-WM983B and the distant lung WM793B-WM793B-1205Lu metastatic models. The majority of metastasis genes were downregulated and enriched in adhesion and ITGA6-B4 pathways. Upregulation of immune pathways was characteristic of distant metastases, whereas increased Rap1 signaling was specific for regional (sub)cutaneous metastases. qRT-PCR analysis of selected integrins (A2, A3, A4, A9, B5, B8, A6, B1, and B3) highlighted the possible importance of ITGA3/4 and B8 in the metastatic process, distinguishing regional and distant metastases. We identified functionally relevant gene clusters that influenced metastasis formation. Our data provide further evidence that integrin expression patterns may be important in distant metastasis formation. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|