Use of mass spectrometry to identify protein biomarkers of disease severity in the synovial fluid and serum of patients with rheumatoid arthritis
| Autor: | Jiang Wu, Johann Karl, Betty Chang, Brad C. Guild, Wendy Chin, Werner Zolg, Fritz Hasler, Hua Liao, Michael D. Jones, Eric Kuhn, Ronenn Roubenoff, Karl R. Clauser, Steve O’Neil |
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| Rok vydání: | 2004 |
| Předmět: |
Adult
Male Proteomics Spectrometry Mass Electrospray Ionization Protein mass spectrometry Immunology Protein Array Analysis S100A9 S100A8 Arthritis Rheumatoid Rheumatology Synovial Fluid Immunology and Allergy Medicine Synovial fluid Humans Pharmacology (medical) Biomarker discovery Chromatography High Pressure Liquid business.industry Selected reaction monitoring Blood Proteins medicine.disease Rheumatoid arthritis Proteome Female business Biomarkers |
| Zdroj: | Arthritis and rheumatism. 50(12) |
| ISSN: | 0004-3591 |
| Popis: | Objective To identify a panel of candidate protein biomarkers of rheumatoid arthritis (RA) that can predict which patients will develop erosive, disabling disease. Methods A 2-step proteomic approach was used for biomarker discovery and verification. In the first step, 2-dimensional liquid chromatography–coupled tandem mass spectrometry was used to generate protein profiles of synovial fluid (SF) from patients with either erosive RA (n = 5) or nonerosive RA (n = 5). In the second step, the selected candidate markers were verified using quantitative multiple reaction monitoring mass spectrometry in sera of patients with erosive RA (n = 15) or nonerosive RA (n = 15) and of healthy controls (n = 15). Results Through differential profiling of proteins in the |
| Databáze: | OpenAIRE |
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