Zika Virus Infects Human Cortical Neural Progenitors and Attenuates Their Growth
| Autor: | Hengli Tang, Emily M. Lee, Jaehoon Shin, Ruth Didier, Guo Li Ming, Bing Yao, Yujing Li, Xuyu Qian, Hongjun Song, Zhexing Wen, Peng Jin, Christy Hammack, Sarah C. Ogden, Feiran Zhang, Kimberly M. Christian |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Programmed cell death Microcephaly Induced Pluripotent Stem Cells Zika virus 03 medical and health sciences Neural Stem Cells Genetics medicine Humans Progenitor cell Induced pluripotent stem cell Cell Proliferation Regulation of gene expression Cell Death biology Zika Virus Infection Cell Cycle Zika Virus Cell Biology biology.organism_classification medicine.disease Virology Neural stem cell Flavivirus 030104 developmental biology Gene Expression Regulation Molecular Medicine |
| Zdroj: | Cell Stem Cell. 18:587-590 |
| ISSN: | 1934-5909 |
| Popis: | SUMMARY The suspected link between infection by Zika virus (ZIKV), a re-emerging flavivirus, and microcephaly is an urgent global health concern. The direct target cells of ZIKV in the developing human fetus are not clear. Here we show that a strain of the ZIKV, MR766, serially passaged in monkey and mosquito cells efficiently infects human neural progenitor cells (hNPCs) derived from induced pluripotent stem cells. Infected hNPCs further release infectious ZIKV particles. Importantly, ZIKV infection increases cell death and dysregulates cell-cycle progression, resulting in attenuated hNPC growth. Global gene expression analysis of infected hNPCs reveals transcriptional dysregulation, notably of cell-cycle-related pathways. Our results identify hNPCs as a direct ZIKV target. In addition, we establish a tractable experimental model system to investigate the impact and mechanism of ZIKV on human brain development and provide a platform to screen therapeutic compounds. |
| Databáze: | OpenAIRE |
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