Mutagenicity assay with Salmonella typhimurium revealing biotransformation of antiphytoviral substances by cell-free plant extract
| Autor: | Gottfried Schuster, Rolf Braun, Adel El-Tarras, Eckart Stenz |
|---|---|
| Rok vydání: | 1989 |
| Předmět: |
Salmonella typhimurium
endocrine system Salmonella Auxotrophy Reversion medicine.disease_cause Antiviral Agents Zea mays Microbiology Plant Viruses chemistry.chemical_compound Mice Biotransformation medicine Animals Histidine biology Mutagenicity Tests Plant Extracts fungi food and beverages General Medicine biology.organism_classification Enterobacteriaceae Biochemistry chemistry Urea General Agricultural and Biological Sciences Liver Extracts Bacteria |
| Zdroj: | Zentralblatt fur Mikrobiologie. 144(3) |
| ISSN: | 0232-4393 |
| Popis: | Summary The mutagenic activity of four antiphytoviral substances was tested in reversion mutagenicity assays with a set of histidine auxotrophic strains of Salmonella typhimurium by means of the preincubation method. A possible metabolic activation of the substances by cell free fractions from maize seedlings (S-14-fraction) and for comparison from mouse liver (S-9 mix) was examined. None of the guanidine, phenyl urea and thiadiazole compounds exerted mutagenic activity in the bacterial strains in experiments without metabolic activation. Cyanoguanidine- and N-phenyl-N-carboxyphenylurea became mutagenic for Salmonella strain TA 98 after metabolic activation by the S-14 plant fraction. Both substances were not mutagenic in the presence of S-9 mix made from mouse liver. The promutagen cyclophosphamide proved highly mutagenic in experiments with S-14 mediated plant metabolic activation. This kind of bacterial mutagenicity assay is valuable in investigations of potential agrochemicals, as the examples have shown. |
| Databáze: | OpenAIRE |
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