Association between HLA‐DRB1*01 and HLA‐DRB1*15 with alloimmunisation in transfusion‐dependent patients with thalassaemia
| Autor: | Zari Tahannejad Asadi, Dian Dayer, Bijan Keikhaei, Mohammad Ali Jalalifar, Mina Ebrahimi |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Adult
Male medicine.medical_specialty 030204 cardiovascular system & hematology Gastroenterology 03 medical and health sciences 0302 clinical medicine Antigen Internal medicine Humans Medicine HLA-DRB1 Genotyping Alleles Membrane Glycoproteins Rh-Hr Blood-Group System business.industry Metalloendopeptidases Transfusion Reaction Hematology Odds ratio Middle Aged Kell antigen system Confidence interval Transplantation Transfusion dependence Thalassemia Female business HLA-DRB1 Chains 030215 immunology |
| Zdroj: | Transfusion Medicine. 30:275-280 |
| ISSN: | 1365-3148 0958-7578 |
| DOI: | 10.1111/tme.12677 |
| Popis: | Background Alloantibody production is one of the most challenging complications in transfusion-dependent thalassaemia patients. Haemolytic anaemia, an increase in blood consumption, difficulty in haematopoietic stem cell transplantation and reduced quality of life are consequences of alloimmunisation. The most predisposed antigens (Ags) for alloantibody development are Rh and Kell blood group Ags. Objective The aim of the present study is to evaluate any correlation between HLA-DRB1 alleles and Rh and Kell alloantibodies. Materials and methods Fifty-two non-responders (control) and 54 responders (case) were enrolled in this study. Alloantibody detection was performed using the tube method. Genotyping of HLA-DRB1*01 and HLA-DRB1*15 was conducted by single-specific primer-polymerase chain reaction. Results In the responder group, 77.8% were hyper-responders (more than one alloantibody), and only 22.2% were mono-responders. Most detected alloantibodies were Anti-K (94.4%), followed by Anti-E (64.8%), Anti-C (29.6%) and Anti-D (25.9%). There was a significant difference in HLA-DRB1*15 between responder and non-responder groups, 73.7% vs 26.3%, respectively. (P = .029, OR = 3.290; 95%CI). Our results showed that HLA-DRB1*15 was more frequent in hyper-responders than mono-responders (92.9% vs 7.1%) (P = .007). The greatest HLA-DRB1*15 was seen in Anti-K (P = .014, odds ratio [OR = 3.784]; 95% confidence interval [CI]) and Anti-E (P = .011, OR = 3.609; 95%CI) alloantibodies. There is no association between HLA-DRB1*01 and alloimmunisation. Conclusion Our findings showed that there is a significant correlation between HLA-DRB1*15 and Anti-K and Anti-E alloantibodies. These findings can be useful in detecting susceptible thalassaemic patients and improving transfusion management. |
| Databáze: | OpenAIRE |
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