Promotion of differentiation in human colon carcinoma cells by vasoactive intestinal polypeptide
| Autor: | Michael G. Brattain, Diane E. Brattain, Naseema M. Hoosein, Brad E. Black |
|---|---|
| Rok vydání: | 1989 |
| Předmět: |
medicine.medical_specialty
Physiology Cellular differentiation Clinical Biochemistry Vasoactive intestinal peptide Peptide hormone Biology Biochemistry Cellular and Molecular Neuroscience Endocrinology Carcinoembryonic antigen Theophylline Internal medicine Cyclic AMP Dibutyryl Cyclic GMP Tumor Cells Cultured medicine Humans Secretion Dose-Response Relationship Drug Colforsin Mucins Cell Differentiation Adenosine Carcinoembryonic Antigen Gastrointestinal hormone Cell culture Colonic Neoplasms biology.protein Cell Division hormones hormone substitutes and hormone antagonists Vasoactive Intestinal Peptide medicine.drug |
| Zdroj: | Regulatory Peptides. 24:15-26 |
| ISSN: | 0167-0115 |
| Popis: | The effects of vasoactive intestinal polypeptide (VIP) and dibutyryl cyclic adenosine 3':5'monophosphate (dbcAMP) on two human colon carcinoma cell lines, HCT 116 and GEO, were investigated. VIP and dbcAMP inhibited the growth of both cell lines in monolayer culture in a dose-dependent manner. Within 6 h of treatment with 1 mM dbcAMP or 0.3 microM VIP, numerous mucin-like droplets were secreted by GEO cells. VIP and dbcAMP also increased carcinoembryonic antigen (CEA) secretion. In both cell lines, a 9-fold increase in conditioned medium CEA levels was observed at 1 mM dbcAMP and a 2.6-fold increase at 1.5 microM VIP. Time- and concentration-dependent evaluation in cAMP levels were elicited by VIP in the two cell lines. Immunocytochemical studies for cell-surface glycoprotein detection in GEO cells showed that VIP induced a morphological and functional organization of mucin-secreting cells. These results indicate that VIP and dbcAMP have antiproliferative and strong differentiation-promoting effects in colon cancer cells. This is the first report of VIP-induced mucin secretion in colon tumor cells. |
| Databáze: | OpenAIRE |
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