Genome-wide identification and expression analysis of theDofgene family under drought stress in tea (Camellia sinensis)
| Autor: | Qian Yu, Yue Zhang, Lixia Zhang, Xiang Qinzeng, Chen Li, Yueyue Tian, Zhengqun Zhang, Hanyue Wang, Jiucheng Zhang, Han Xiaoyang |
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| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
Drought stress lcsh:Medicine Biology 01 natural sciences Genome General Biochemistry Genetics and Molecular Biology 03 medical and health sciences Gene expression Gene family Camellia sinensis Gene 030304 developmental biology Genetics Zinc finger 0303 health sciences Tea Phylogenetic tree Abiotic stress General Neuroscience fungi lcsh:R food and beverages General Medicine Dof transcription factors General Agricultural and Biological Sciences 010606 plant biology & botany |
| Zdroj: | PeerJ, Vol 8, p e9269 (2020) |
| ISSN: | 2167-8359 |
| Popis: | BackgroundDNA-binding one zinc finger (Dof) proteins are plant-specific transcription factors important for seed development, hormone regulation, and defense against abiotic stress. Although drought stress is a key determinant of plant physiology and metabolic homeostasis, the role ofDofgenes in different degrees of PEG6000-induced drought stress has received little attention.MethodsTea plants (Camellia sinensis) were exposed to mild, moderate and severe drought stress. The Tea Genome and Plant TFDB databases were used to identifyDofgene family members in the tea plant. Clustal W2.1, MEGA6.0, ScanProsite, SMART, ExPASy, GSDS, MEME and STRING were used to build a phylogenetic tree, predict the molecular masses and isoelectric points of the Dof proteins, and construct a predicted protein-protein interaction network between the CsDof TFs and proteins in theA. thalianadatabase. The expression patterns ofDofgenes in different tissues were analyzed, and qRT-PCR was used to measure the expression ofDofgenes under different degrees of drought stress in tea.ResultsWe identified 16Dofgenes in tea (C. sinensiscv. Huangjinya) using whole-genome analysis. Through comparative analysis of tea andArabidopsis thaliana, we divided theDofgenes into four families (A, B, C, and D). We identified 15 motifs in the amino acid sequences of the CsDof proteins. Gene sequences and motif structures were highly conserved among families, especially in the B1 and C2 subfamilies. The protein-protein interaction network indicated that multiple CsDof proteins may be involved in the response to drought stress. Real-time PCR was used to examine the tissue-specific expression patterns of theCsDofgenes and to measure their responses to different levels of PEG6000-induced drought stress in mature leaves. MostCsDofgenes responded to drought stress. These results provide information on theDofgene family in tea, offer new insights into the function ofCsDofgenes in a perennial species, and lay the foundation for further analysis of their functions. |
| Databáze: | OpenAIRE |
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