OsBIC1 Directly Interacts with OsCRYs to Regulate Leaf Sheath Length through Mediating GA-Responsive Pathway
| Autor: | Cong Li, Xin Wang, Liya Zhang, Chunyu Zhang, Chunsheng Yu, Tao Zhao, Bin Liu, Hongyu Li, Jun Liu |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
cryptochrome (CRY)
Light QH301-705.5 blue-light inhibitor of cryptochrome (BIC) Article Catalysis Plant Epidermis Inorganic Chemistry Gene Expression Regulation Plant leaf sheath length Biology (General) Physical and Theoretical Chemistry QD1-999 Molecular Biology Spectroscopy Plant Proteins blue light GA fungi Organic Chemistry food and beverages Oryza General Medicine Gibberellins Computer Science Applications Plant Leaves Chemistry Transcriptome Protein Binding |
| Zdroj: | International Journal of Molecular Sciences; Volume 23; Issue 1; Pages: 287 International Journal of Molecular Sciences International Journal of Molecular Sciences, Vol 23, Iss 287, p 287 (2022) |
| ISSN: | 1422-0067 |
| DOI: | 10.3390/ijms23010287 |
| Popis: | Cryptochrome 1 and 2 (CRY1 and CRY2) are blue light receptors involved in the regulation of hypocotyl elongation, cotyledon expansion, and flowering time in Arabidopsisthaliana. Two cryptochrome-interacting proteins, Blue-light Inhibitor of Cryptochrome 1 and 2 (BIC1 and BIC2), have been found in Arabidopsis. BIC1 plays critical roles in suppressing the physiological activities of CRY2, which include the blue light-dependent dimerization, phosphorylation, photobody formation, and degradation process, but the functional characterization of BIC protein in other crops has not yet been performed. To investigate the function of BIC protein in rice (Oryza sativa), two homologous genes of Arabidopsis BIC1 and BIC2, namely OsBIC1 and OsBIC2 (OsBICs), were identified. The overexpression of OsBIC1 and OsBIC2 led to increased leaf sheath length, whereas mutations in OsBIC1 displayed shorter leaf sheath in a blue light intensity-dependent manner. OsBIC1 regulated blue light-induced leaf sheath elongation through direct interaction with OsCRY1a, OsCRY1b, and OsCRY2 (OsCRYs). Longitudinal sections of the second leaf sheath demonstrated that OsBIC1 and OsCRYs controlled leaf sheath length by influencing the ratio of epidermal cells with different lengths. RNA-sequencing (RNA-seq) and quantitative Real-Time Polymerase Chain Reaction (qRT-PCR) analysis further proved that OsBIC1 and OsCRYs regulated similar transcriptome changes in regulating Gibberellic Acids (GA)-responsive pathway. Taken together, these results suggested that OsBIC1 and OsCRYs worked together to regulate epidermal cell elongation and control blue light-induced leaf sheath elongation through the GA-responsive pathway. |
| Databáze: | OpenAIRE |
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