Characterizing uncommon Burkholderia cepacia complex isolates from an outbreak in a haemodialysis unit
| Autor: | Viviane Cristina Caetano, Patrícia Severino, Cláudia R. Moreira, Fátima M. Azevedo, Peter Vandamme, Vanda D. Magalhães, Denise Alves Saltini, Andrea Vieira de Souza, Suely Itsuko Ciosak, Jacyr Pasternak, Maria de Lurdes Hirata |
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| Rok vydání: | 2004 |
| Předmět: |
Microbiology (medical)
Burkholderia cepacia Integron Microbiology Ribotyping Disease Outbreaks Integrons Genotype Humans Cross Infection biology Outbreak Genomovar Burkholderia Infections General Medicine Gel electrophoresis of proteins biology.organism_classification RAPD Random Amplified Polymorphic DNA Technique Burkholderia cepacia complex Hemodialysis Units Hospital biology.protein bacteria Water Microbiology |
| Zdroj: | Journal of medical microbiology. 53(Pt 10) |
| ISSN: | 0022-2615 |
| Popis: | An outbreak of bacteraemia in a haemodialysis unit where 65 episodes of infection involved 35 outpatients is reported. Burkholderia cepacia complex was the agent most frequently recovered from blood. Thirty-three environmental and clinical isolates of B. cepacia complex were characterized by whole-cell protein electrophoresis and recA-RFLP profile. Fourteen isolates were genomovar I and 16 isolates were not classifiable by their recA-RFLP pattern. Ribotyping, random amplification of polymorphic DNA (RAPD) and integron profile were used to explore the clonality of the isolates, and revealed multiple strain genotypes. Four ribotypes and RAPD types and three integron patterns were identified. The water supply was identified as the source of the outbreak, and inappropriate cleaning and a leak in the reverse osmosis tubing connection were the probable causes of contamination. B. cepacia complex was still recovered from blood of patients even after apparently adequate measures were taken and water quality standards were met, suggesting that higher standards for water quality should be adopted in haemodialysis units. The genomovars recovered here were distinct from those commonly reported for cystic fibrosis isolates. |
| Databáze: | OpenAIRE |
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