Identification of an orphan guanylate cyclase receptor selectively expressed in mouse testis
| Autor: | Danuta Mitko, Michaela Kuhn, Ruey-Bing Yang, Nga Bien-Ly, László G Kömüves, Chi Kin Domingos Ng, Ana Kilic, Yueh-Hsing Su |
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| Jazyk: | angličtina |
| Rok vydání: | 2004 |
| Předmět: |
Male
Glycosylation Molecular Sequence Data Gene Expression Receptors Cell Surface Biology Biochemistry Cyclase Cell Line Mice Testis Animals Humans Tissue Distribution Amino Acid Sequence Cloning Molecular Receptor Natriuretic Peptides Molecular Biology Cyclic GMP Phylogeny HEK 293 cells Membrane Proteins Cell Biology Guanylate cyclase 2C NPR1 NPR2 Molecular biology Transmembrane protein Guanylate Cyclase GUCY2D Research Article |
| Popis: | We have identified a novel membrane form of guanylate cyclase (GC) from a mouse testis cDNA library and termed it mGC-G (mouse GC-G) based on its high sequence homology to rat GC-G. It encodes a potential type I transmembrane receptor, with the characteristic domain structure common to all members of the family of membrane GCs, including an extracellular, putative ligand-binding domain, a single membrane-spanning segment and cytoplasmic protein kinase-like and cyclase catalytic domains. Real-time quantitative reverse transcriptase–PCR and Northern-blot analyses showed that mGC-G is highly and selectively expressed in mouse testis. Phylogenetic analysis based on the extracellular protein sequence revealed that mGC-G is closely related to members of the subfamily of natriuretic peptide receptor GCs. When overexpressed in HEK-293T cells (human embryonic kidney 293T cells) or COS-7 cells, mGC-G manifests as a membrane-bound glycoprotein, which can form either homomeric or heteromeric complexes with the natriuretic peptide receptor GC-A. It exhibits marked cGMP-generating GC activity; however, notably, all ligands known to activate other receptor GCs failed to stimulate enzymic activity. The unique testis-enriched expression of mGC-G, which is completely different from the broader tissue distribution of rat GC-G, suggests the existence of as-yet-unidentified ligands and unappreciated species-specific physiological functions mediated through mGC-G/cGMP signalling in the testis. |
| Databáze: | OpenAIRE |
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