Proteome and phosphoproteome profiling reveals the regulation mechanism of hibernation in a freshwater leech (Whitmania pigra)
Autor: | Hong-Zhuan Shi, Shi-Meng Yan, Zaibiao Zhu, Xiangjing Hu, Qiao-Sheng Guo, Dao-Xin Dai, Yiming Lu, Xibin Yang, Fei Liu, Jia Wang |
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Rok vydání: | 2019 |
Předmět: |
0301 basic medicine
Proteomics animal structures 030102 biochemistry & molecular biology Proteome Quantitative proteomics Biophysics Whitmania pigra Zoology Leech Fresh Water Biology Tandem mass tag Biochemistry 03 medical and health sciences Isobaric labeling 030104 developmental biology Ectotherm Hibernation Leeches Animals Protein phosphorylation |
Zdroj: | Journal of proteomics. 229 |
ISSN: | 1876-7737 |
Popis: | Hibernation is an energy-saving and adaptive strategy adopted by leech, an important medicinal resource in Asia, to survive low temperature. Reversible protein phosphorylation (RPP) plays a key role in the regulation of mammalian hibernation processes but has never been documented in freshwater invertebrate such as leech. In this study, we detected the effects of hibernation on the proteome and phosphoproteome of the leech Whitmania pigra. A total of 2184 proteins and 2598 sites were quantified. Deep-hibernation resulted in 85 up-regulated and 107 down-regulated proteins and 318 up-regulated and 204 down-regulated phosphosites using a 1.5-fold threshold (P0.05). Proteins involved in protein digestion and absorption, amino acid metabolism and N-glycan biosynthesis were significantly down-regulated during deep-hibernation. However, proteins involved in maintaining cell structure stability in hibernating animals were up-regulated. Differentially phosphorylated proteins provided the first global picture of a shift in energy metabolism, protein synthesis, cytoprotection and signaling during deep hibernation. Furthermore, AMP-activated protein kinase and protein kinase C play major roles in the regulation of these functional processes. These data significantly improve our understanding of the regulatory mechanisms of leech hibernation processes and provides substantial candidate phosphorylated proteins that could be important for functionally adapt in freshwater animals. SIGNIFICANCE: The leech Whitmania pigra as an important medicinal resource in Asia is an excellent model freshwater invertebrate for studies of environmentally-induced hibernation. The present study provides the first quantitative proteomics and phosphoproteomic analysis of leech hibernation using isobaric tag based TMT labeling and high-resolution mass spectrometry. These data significantly improve our understanding of the regulatory mechanisms when ectotherm animals face environmental stress and provides substantial candidate phosphorylated proteins that could be important for functionally adapt in freshwater animals. |
Databáze: | OpenAIRE |
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