Enhancing effect of chitosan on peptide drug delivery across buccal mucosa
| Autor: | Christopher A. Squier, Mary J. Kremer, Philip W. Wertz, S Kaş, A. Atilla Hincal, Sevda Şenel |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Drug
Swine Bioadhesive media_common.quotation_subject medicine.medical_treatment Biophysics Chitin Bioengineering Peptide Pharmacology Biology Biomaterials Chitosan chemistry.chemical_compound Transforming Growth Factor beta medicine Animals Oral mucosa media_common chemistry.chemical_classification Protease Mouth Mucosa Lactic acid medicine.anatomical_structure Biochemistry chemistry Mechanics of Materials Drug delivery Ceramics and Composites |
| Zdroj: | Biomaterials. 21:2067-2071 |
| ISSN: | 0142-9612 |
| DOI: | 10.1016/s0142-9612(00)00134-4 |
| Popis: | The buccal mucosa represents a potentially important topical route for delivery of peptide or protein drugs with some unique advantages such as the avoidance of hepatic first-pass metabolism and the acidity and protease activity encountered in the gastrointestinal tract. However, the bioavailabilities or relative potencies of intraorally administered peptides are usually quite low, unless permeabilizers are employed. Chitosan, a mucopolysaccharide of marine origin, has been claimed to act both as a bioadhesive and permeabilizer, making it a candidate system for mucosal drug delivery. In this study, the enhancement effect of chitosan in gel form for oral mucosa was investigated with a large bioactive peptide, transforming growth factor-beta (TGF-beta). Chitosan gel was prepared at 2% concentration in dilute lactic acid and TGF-beta was incorporated into the gel. The effect of chitosan as a permeabilizer was determined by measuring the flux of TGF-beta across porcine oral mucosa in an in vitro system. The localization of TGF-beta within the oral mucosa was determined by horizontal sectioning and counting. Chitosan was found to exert a marked permeabilizing effect on buccal mucosa for peptide drug. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect