Static Magnetic Field Attenuates Lipopolysaccharide-Induced Inflammation in Pulp Cells by Affecting Cell Membrane Stability
| Autor: | Yung Kai Huang, Jeng Ting Tsao, Haw Ming Huang, Ya Hui Chan, Che Tong Lin, Lin Wen Lee, Wei Zhen Lew, Sung Chih Hsieh |
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| Rok vydání: | 2015 |
| Předmět: |
Lipopolysaccharides
Pathology medicine.medical_specialty Article Subject Lipopolysaccharide Cell Survival Population lcsh:Medicine Fluorescence Polarization Inflammation lcsh:Technology General Biochemistry Genetics and Molecular Biology chemistry.chemical_compound stomatognathic system Dental pulp stem cells Humans Medicine MTT assay Viability assay lcsh:Science education Cells Cultured Dental Pulp Cell Proliferation General Environmental Science education.field_of_study Staining and Labeling lcsh:T business.industry Cell Membrane lcsh:R General Medicine Flow Cytometry Cell biology stomatognathic diseases Magnetic Fields Odontoblast chemistry Pulp (tooth) lcsh:Q medicine.symptom business Research Article |
| Zdroj: | The Scientific World Journal, Vol 2015 (2015) The Scientific World Journal |
| ISSN: | 1537-744X 2356-6140 |
| Popis: | One of the causes of dental pulpitis is lipopolysaccharide- (LPS-) induced inflammatory response. Following pulp tissue inflammation, odontoblasts, dental pulp cells (DPCs), and dental pulp stem cells (DPSCs) will activate and repair damaged tissue to maintain homeostasis. However, when LPS infection is too serious, dental repair is impossible and disease may progress to irreversible pulpitis. Therefore, the aim of this study was to examine whether static magnetic field (SMF) can attenuate inflammatory response of dental pulp cells challenged with LPS. In methodology, dental pulp cells were isolated from extracted teeth. The population of DPSCs in the cultured DPCs was identified by phenotypes and multilineage differentiation. The effects of 0.4 T SMF on DPCs were observed through MTT assay and fluorescent anisotropy assay. Our results showed that the SMF exposure had no effect on surface markers or multilineage differentiation capability. However, SMF exposure increases cell viability by 15%. In addition, SMF increased cell membrane rigidity which is directly related to higher fluorescent anisotropy. In the LPS-challenged condition, DPCs treated with SMF demonstrated a higher tolerance to LPS-induced inflammatory response when compared to untreated controls. According to these results, we suggest that 0.4 T SMF attenuates LPS-induced inflammatory response to DPCs by changing cell membrane stability. |
| Databáze: | OpenAIRE |
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