The malignant histiocytosis sarcoma virus, a recombinant of Harvey murine sarcoma virus and Friend mink cell focus-forming virus, has acquired myeloid transformation specificity by alterations in the long terminal repeat
| Autor: | J Nowock, D Hughes, Jutta Friel, Wolfram Ostertag, R A Padua, Carol Stocking, C Laker, I Pragnell |
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| Rok vydání: | 1990 |
| Předmět: |
Genes
Viral viruses Molecular Sequence Data Restriction Mapping Immunology Harvey murine sarcoma virus Viral transformation Biology Transfection Microbiology Virus Cell Line Sarcoma Viruses Murine Mice Viral Envelope Proteins Mink Cell Focus-Inducing Viruses Sequence Homology Nucleic Acid Virology Murine leukemia virus Animals Direct repeat Amino Acid Sequence Cloning Molecular Codon Repetitive Sequences Nucleic Acid Recombination Genetic Mice Inbred BALB C Base Sequence Point mutation Provirus biology.organism_classification Long terminal repeat Leukemia Virus Murine Cell Transformation Neoplastic Genes ras Insect Science DNA Viral Histiocytosis Spleen Research Article |
| Zdroj: | Journal of Virology. 64:369-378 |
| ISSN: | 1098-5514 0022-538X |
| DOI: | 10.1128/jvi.64.1.369-378.1990 |
| Popis: | The malignant histiocytosis sarcoma virus (MHSV), in contrast to other viruses with the ras oncogene, induces acute histiocytosis in newborn and adult mice. Molecular structure and function studies were initiated to determine the basis of its unique macrophage-transforming potential. Characterization of the genomic structure showed that the virus evolved by recombination of the Harvey murine sarcoma virus (Ha-MuSV) and a virus of the Friend-mink cell focus-forming virus family. Structural analysis of MHSV showed two regions of the genome that are basically different from the Ha-MuSV: (i) the ras gene, which is altered by a point mutation in codon 181 leading to a Cys----Ser substitution of the p21 protein, and (ii) the U3 region of the long terminal repeat, which is largely derived from F-MCFV and contains a deletion of one direct repeat as well as a duplication of an altered enhancer-like region. Biological studies of Ha-MuSV, MHSV, and recombinants between the two viruses show that the U3 region of the MHSV long terminal repeat is essential for the malignancy and specificity of the disease. A contributing role of the ras point mutation in determining macrophage specificity, however, cannot be excluded. |
| Databáze: | OpenAIRE |
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