Regulation of translation factors during hindlimb unloading and denervation of skeletal muscle in rats
| Autor: | R. Bridge Hunter, Karyn A. Esser, Susan C. Kandarian, Troy A. Hornberger |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Elongation Factor 2 Kinase
Physiology Eukaryotic Initiation Factor-2 Immunoblotting Hindlimb Biology Protein degradation Eukaryotic translation medicine Protein biosynthesis Initiation factor Animals Phosphorylation Rats Wistar Muscle Skeletal Denervation Ribosomal Protein S6 Kinases Skeletal muscle Cell Biology musculoskeletal system Muscle atrophy Cell biology Rats Muscular Atrophy medicine.anatomical_structure Biochemistry Hindlimb Suspension Protein Biosynthesis Calcium-Calmodulin-Dependent Protein Kinases Female medicine.symptom |
| Zdroj: | Europe PubMed Central |
| ISSN: | 0363-6143 |
| Popis: | In the rat, denervation and hindlimb unloading are two commonly employed models used to study skeletal muscle atrophy. In these models, muscle atrophy is generally produced by a decrease in protein synthesis and an increase in protein degradation. The decrease in protein synthesis has been suggested to occur by an inhibition at the level of protein translation. To better characterize the regulation of protein translation, we investigated the changes that occur in various translation initiation and elongation factors. We demonstrated that both hindlimb unloading and denervation produce alterations in the phosphorylation and/or total amount of the 70-kDa ribosomal S6 kinase, eukaryotic initiation factor 2 α-subunit, and eukaryotic elongation factor 2. Our findings indicate that the regulation of these protein translation factors differs between the models of atrophy studied and between the muscles evaluated (e.g., soleus vs. extensor digitorum longus). |
| Databáze: | OpenAIRE |
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