Discovery of genes with highly restricted expression patterns in theDrosophilawing disc using DNA oligonucleotide microarrays
| Autor: | Roger Phillips, Thomas L Jacobsen, Donna Cain, Miranda J. Butler, J. Robert S. Whittle, Michael G. Jarman, Amanda Simcox, Mike Hubank |
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| Rok vydání: | 2003 |
| Předmět: |
Cell type
animal structures Oligonucleotides Wnt1 Protein In situ hybridization Biology Animals Genetically Modified DNA Microarray Analysis Proto-Oncogene Proteins Animals Drosophila Proteins Wings Animal Eye Proteins Molecular Biology Transcription factor Gene In Situ Hybridization Oligonucleotide Array Sequence Analysis Homeodomain Proteins Regulation of gene expression Genetics Wing Twist-Related Protein 1 Gene Expression Regulation Developmental Nuclear Proteins Larva POU Domain Factors Drosophila Drosophila Protein Signal Transduction Transcription Factors Developmental Biology |
| Zdroj: | Development. 130:659-670 |
| ISSN: | 1477-9129 0950-1991 |
| DOI: | 10.1242/dev.00293 |
| Popis: | The Drosophila wing disc is divided along the proximaldistal axis into regions giving rise to the body wall (proximal), wing hinge(central) and wing blade (distal). We applied DNA microarray analysis to discover genes with potential roles in the development of these regions. We identified a set of 94 transcripts enriched (two fold or greater) in the body wall and 56 transcripts enriched in the wing/hinge region. Transcripts that are known to have highly restricted expression patterns, such aspannier, twist and Bar-H1 (body wall) and knot,nubbin and Distal-less (wing/hinge), showed strong differential expression on the arrays. In situ hybridization for 50 previously uncharacterized genes similarly revealed that transcript enrichment identified by the array analysis was consistent with the observed spatial expression. There was a broad spectrum of patterns, in some cases suggesting that the genes could be targets of known signaling pathways. We show that three of these genes respond to wingless signaling. We also discovered genes likely to play specific roles in tracheal and myoblast cell types, as these cells are part of the body wall fragment. In summary, the identification of genes with restricted expression patterns using whole genome profiling suggests that many genes with potential roles in wing disc development remain to be characterized. |
| Databáze: | OpenAIRE |
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