Epidermal growth factor reduces ischemia-reperfusion injury in rat small intestine
Autor: | Charles L. Schleien, Xavier Villa, John F. Thompson, John W. Kuluz |
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Rok vydání: | 2002 |
Předmět: |
Male
Pathology medicine.medical_specialty Ischemia Critical Care and Intensive Care Medicine Permeability Rats Sprague-Dawley Jejunum Intestinal mucosa Epidermal growth factor Internal medicine medicine.artery Intestine Small medicine Animals Superior mesenteric artery Intestinal Mucosa Intestinal permeability Epidermal Growth Factor business.industry medicine.disease Small intestine Rats medicine.anatomical_structure Endocrinology Reperfusion Injury business Reperfusion injury |
Zdroj: | Critical Care Medicine. 30:1576-1580 |
ISSN: | 0090-3493 |
Popis: | Objective: To measure the effect of pre-ischemic administration of intraluminal epidermal growth factor on the changes in intestinal permeability induced by 30 mins of superior mesenteric artery occlusion, followed by 2 hrs of reperfusion. Design: Prospective, randomized, placebo-controlled experimental study. Setting: University basic science research laboratory. Subjects: Healthy, young, adult, male Sprague-Dawley rats. Interventions: A 10-cm segment of small intestine was isolated and studied in situ in rats that were anesthetized with fentanyl and mechanically ventilated. Intestinal ischemia-reperfusion injury was induced by temporary occlusion of the superior mesenteric artery for 30 mins, followed by 2 hrs of reperfusion. Three groups were studied: time controls with a sham operation, saline-treated ischemia-reperfusion, and epidermal growth factor-treated ischemia-reperfusion. Epidermal growth factor, 100 ng/ min, was infused intraluminally, beginning 30 mins before and continued until 40 mins after ischemia. Measurements and Main Results: Intestinal permeability was measured for each 10-min time period by using chromium-labeled EDTA. Histopathologic injury was assessed by light microscopy. After superior mesenteric artery occlusion, intestinal permeability increased approximately ten-fold and was sustained for 2 hrs of reperfusion in saline-treated rats. Pretreatment with epidermal growth factor significantly reduced the permeability changes during reperfusion by >60% compared with saline-treated animals (p |
Databáze: | OpenAIRE |
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