A novel Streptomyces restriction endonuclease, Sse1825I, cleaving at 5'-GG/GWCCC-3'
| Autor: | Ikunoshin Kato, Fusao Kimizuka, Yoshiko Nomura, Yoshizumi Ishino |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
chemistry.chemical_classification
biology Base Sequence viruses Molecular Sequence Data General Medicine DNA biology.organism_classification Molecular biology Streptomyces PBR322 Restriction fragment Substrate Specificity chemistry.chemical_compound Restriction enzyme Enzyme chemistry Oligodeoxyribonucleotides Cleave Genetics biology.protein pUC19 Deoxyribonucleases Type II Site-Specific |
| Zdroj: | Gene. 157(1-2) |
| ISSN: | 0378-1119 |
| Popis: | We isolated and characterized from a Streptomyces species a new class-II restriction endonuclease, which recognizes the palindromic heptanucleotide sequence 5′-G G↓G W C C C 3′-C C C W G↑G G (where W = A or T) and cleaves double-stranded DNA after the second G in this sequence This Sse18251 enzyme cleaves phage λ DNA at one site, adenovirus type 2 DNA at eight sites, but does not cleave pBR322, SV40, CoIE1, pUC18 and pUC19, and replicative forms of M13mpl8 and M13mpl9, and ΦX174 DNAs. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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