A novel glucose 6-phosphate isomerase from Listeria monocytogenes
| Autor: | Jeffrey M. Schaub, Tod P. Holler, M.C. Holt, David L. Cech, Pan Fen Wang, Ronald W. Woodard, Victoria A. Assimon |
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| Rok vydání: | 2014 |
| Předmět: |
Molecular Sequence Data
Bioengineering Isomerase Biology medicine.disease_cause Biochemistry Analytical Chemistry Microbiology law.invention chemistry.chemical_compound Biosynthesis Listeria monocytogenes law medicine Amino Acid Sequence Escherichia coli Aldose-Ketose Isomerases chemistry.chemical_classification Escherichia coli Proteins Organic Chemistry Glucose-6-Phosphate Isomerase Recombinant Proteins Molecular Weight Enzyme chemistry Glucose 6-phosphate Recombinant DNA Bacterial outer membrane Sequence Alignment |
| Zdroj: | The protein journal. 33(5) |
| ISSN: | 1875-8355 |
| Popis: | d-Arabinose 5-phosphate isomerases (APIs) catalyze the interconversion of d-ribulose 5-phosphate and d-arabinose 5-phosphate (A5P). A5P is an intermediate in the biosynthesis of 3-deoxy-d-manno-octulosonate (Kdo), an essential component of lipopolysaccharide, the lipopolysaccharide found in the outer membrane of Gram-negative bacteria. The genome of the Gram-positive pathogen Listeria monocytogenes contains a gene encoding a putative sugar isomerase domain API, Q723E8, with significant similarity to c3406, the only one of four APIs from Escherichia coli CFT073 that lacks a cystathionine-β-synthase domain. However, L. monocytogenes lacks genes encoding any of the other enzymes of the Kdo biosynthesis pathway. Realizing that the discovery of an API in a Gram-positive bacterium could provide insight into an alternate physiological role of A5P in the cell, we prepared and purified recombinant Q723E8. We found that Q723E8 does not possess API activity, but instead is a novel GPI (d-glucose 6-phosphate isomerase). However, the GPI activity of Q723E8 is weak compared with previously described GPIs. L. monocytogenes contains an ortholog of the well-studied two-domain bacterial GPI, so this maybe redundant. Based on this evidence glucose utilization is likely not the primary physiological role of Q723E8. |
| Databáze: | OpenAIRE |
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