Efficient gene transfer into normal human skeletal cells using recombinant adenovirus and conjugated adenovirus-DNA complexes
| Autor: | Pamela Gehron Robey, Beatrice Sommer, R. J. Cristiano, M. F. Young, Brian O'Connell, Sergei A. Kuznetsov |
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| Rok vydání: | 1998 |
| Předmět: |
viruses
Endocrinology Diabetes and Metabolism Genetic Vectors Biology Genome Virus Bone and Bones law.invention Adenoviridae chemistry.chemical_compound Endocrinology law Genes Reporter Humans Orthopedics and Sports Medicine Cells Cultured Reporter gene Gene Transfer Techniques DNA Fibroblasts Molecular biology chemistry Cell culture Polylysine Recombinant DNA Exogenous DNA Stromal Cells |
| Zdroj: | Calcified tissue international. 64(1) |
| ISSN: | 0171-967X |
| Popis: | In order to assess efficient DNA gene transfer into human primary cell cultures derived from the skeleton we tested two viral-based procedures. First, replication-deficient recombinant adenoviruses (ADV) were used to infect post-confluent human marrow stromal fibroblasts (HMSF) and human trabecular bone (HTB) cells. Both cell types were readily infected by modified adenoviral vectors carrying a reporter gene making this virus an attractive candidate to facilitate DNA gene transfer. In a second approach we coincubated DNA with ADV that had polylysine (PLL) covalently attached. With this ADV/PLL/DNA complex, very efficient gene transfer into multilayered HMSF and HTB cell cultures was observed, and DNA coincubated with unmodified ADV failed to be effectively transferred. These data imply that the covalently bound PLL more effectively binds exogenous DNA, resulting in a highly efficient internalization event in both cell types. Thus, this latter method has many advantages over conventional ADV gene transfer procedures. It is simple, rapid, and it does not require engineering of DNA into the viral genome, thereby allowing transfer of large fragments of DNA. |
| Databáze: | OpenAIRE |
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