Collagenase-1 injection improved tumor distribution and gene expression of cationic lipoplex
| Autor: | Yoshie Maitani, Yoshiyuki Hattori, Manami Kubo, Mako Kato |
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| Rok vydání: | 2012 |
| Předmět: |
Time Factors
Pharmaceutical Science Gene delivery Transfection Collagen Type I Carcinoma Lewis Lung Mice Luciferases Firefly Cell Line Tumor Gene expression Pressure medicine Animals Distribution (pharmacology) Tissue Distribution Cationic liposome Collagenases Chemistry Extracellular Fluid DNA Genetic Therapy Molecular biology Mice Inbred C57BL Microbial Collagenase Cell culture Injections Intravenous Collagenase Female Nanocarriers Clostridium histolyticum Type I collagen medicine.drug |
| Zdroj: | International Journal of Pharmaceutics. 423:428-434 |
| ISSN: | 0378-5173 |
| Popis: | Elevated interstitial fluid pressure (IFP) in a tumor is a barrier to tumor accumulation of systemic delivery of nanocarriers. In this study, we investigated whether intravenous injection of type I collagenase (collagenase-1) reduced IFP in tumors and increased the accumulation and gene expression of cationic liposome/plasmid DNA complex (lipoplex) in tumors after intravenous injection into mice bearing mouse lung carcinoma LLC tumors. Collagenase-1 reduced the amount of type I collagen in the tumor, and significantly decreased IFP by 65% at 1h after injection. Therefore, collagenase-1 induced 1.5-fold higher accumulation and 2-fold higher gene expression of lipoplex in tumors after intravenous injection. These findings indicated that intravenous injection of collagenase-1 improved the accumulation of lipoplex by decreasing IFP in tumors. These results support the potential use of collagen digestion as a strategy to improve systemic gene delivery into tumors. |
| Databáze: | OpenAIRE |
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