Biotransformations of Bile Acids with Bacteria from Cayambe Slaughterhouse (Ecuador): Synthesis of Bendigoles
| Autor: | Elena Tamburini, Morena De Bastiani, Paola Pedrini, Alessandro Medici, Maria Elena Maldonado Rodriguez, Stefania Costa, Irene Rugiero |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Molecular Conformation Bioengineering Biotranformations Bile acids Pseudomonas Rhodococcus Bendigoles Hyodeoxycholic acid Biochemistry Bile Acids and Salts 03 medical and health sciences chemistry.chemical_compound Biotransformation Biosynthesis Pseudomonas Organic chemistry Rhodococcus Molecular Biology Hydroxysteroids Chromatography biology Deoxycholic acid Cholic acid Ambientale General Chemistry General Medicine biology.organism_classification Bendigoles Bile acids 030104 developmental biology chemistry Molecular Medicine Biotranformations Ecuador Bacteria Abattoirs |
| Zdroj: | Chemistrybiodiversity. 13(8) |
| ISSN: | 1612-1880 |
| Popis: | The biotransformations of cholic acid (1a), deoxycholic acid (1b), and hyodeoxycholic acid (1c) to bendigoles and other metabolites with bacteria isolated from the rural slaughterhouse of Cayambe (Pichincha Province, Ecuador) were reported. The more active strains were characterized, and belong to the genera Pseudomonas and Rhodococcus. Various biotransformation products were obtained depending on bacteria and substrates. Cholic acid (1a) afforded the 3-oxo and 3-oxo-4-ene derivatives 2a and 3a (45% and 45%, resp.) with P. mendocina ECS10, 3,12-dioxo-4-ene derivative 4a (60%) with Rh. erythropolis ECS25, and 9,10-secosteroid 6 (15%) with Rh. erythropolis ECS12. Bendigole F (5a) was obtained in 20% with P. fragi ECS22. Deoxycholic acid (1b) gave 3-oxo derivative 2b with P. prosekii ECS1 and Rh. erythropolis ECS25 (20% and 61%, resp.), while 3-oxo-4-ene derivative 3b was obtained with P. prosekii ECS1 and P. mendocina ECS10 (22% and 95%, resp.). Moreover, P. fragi ECS9 afforded bendigole A (8b; 80%). Finally, P. mendocina ECS10 biotransformed hyodeoxycholic acid (1c) to 3-oxo derivative 2c (50%) and Rh. erythropolis ECS12 to 6α-hydroxy-3-oxo-23,24-dinor-5β-cholan-22-oic acid (9c, 66%). Bendigole G (5c; 13%) with P. prosekii ECS1 and bendigole H (8c) with P. prosekii ECS1 and Rh. erythropolis ECS12 (20% and 16%, resp.) were obtained. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text