Effects of different sterilization methods of herbal formula on phytochemical compounds and antibacterial activity against mastitis-causing bacteria
| Autor: | Vincentia Rizke Ciptaningtyas, Fajar Wahyono, Dian Wahyu Harjanti |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
medicine.drug_class Veterinary medicine Antibiotics mastitis phytochemical compound SF1-1100 03 medical and health sciences herbal extract SF600-1100 medicine sterilization method Agar diffusion test Curcuma phytobiotic General Veterinary biology Traditional medicine Chemistry 0402 animal and dairy science food and beverages 04 agricultural and veterinary sciences Sterilization (microbiology) biology.organism_classification medicine.disease 040201 dairy & animal science Animal culture Mastitis Penicillin 030104 developmental biology Phytochemical Antibacterial activity Research Article medicine.drug |
| Zdroj: | Veterinary World, Vol 13, Iss 6, Pp 1187-1192 (2020) Veterinary World |
| ISSN: | 2231-0916 0972-8988 |
| DOI: | 10.14202/vetworld.2020.1187-1192 |
| Popis: | Background and Aim: The current phytobiotic industry is struggling to determine a proper sterilization method for an herbal formula that comprises multiple plant extracts. Hence, this study aims to investigate the effects of two sterilization methods of herbal formula on phytochemical compounds and antibacterial activity against mastitis-causing bacterial isolates. Materials and Methods: The herbal formula comprised the extracts of Piper betle leaves, Curcuma domestica, and Curcuma zanthorriza. We applied two sterilization methods – sterilization using 0.45 μM syringe filter and sterilization using an autoclave at 121°C for 15 min. After sterilization, we measured phytochemical contents (phenol and flavonoid) of the herbal formula. Using the disk diffusion method, the antibacterial susceptibility test of the sterilized herbal formula against mastitis-causing bacterial isolates was conducted. Tetracycline, erythromycin, and penicillin – common antibiotics for mastitis therapy in dairy farms – were used as standard antibiotics. Results: Compared with autoclave sterilization, syringe filter sterilization resulted in less (p |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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