A transcriptional activator, AoXlnR, controls the expression of genes encoding xylanolytic enzymes in Aspergillus oryzae

Autor: Jaap Visser, Satoshi Mimura, Leo H. de Graaff, Masashi Kato, Akimitsu Tanaka, Noriyuki Kitamoto, Norihiro Tsukagoshi, Junichiro Marui, Tetsuo Kobayashi
Jazyk: angličtina
Rok vydání: 2002
Předmět:
AFSG Stafafdelingen (WUATV)
Aspergillus oryzae
reductase gene
Sequence Homology
shoyu koji mold
law.invention
chemistry.chemical_compound
law
Microbiologie
Gene Expression Regulation
Fungal
sequence-analysis
Cloning
Molecular
Promoter Regions
Genetic
xylanase genes
chemistry.chemical_classification
Genetics
biology
Amino acid
Xylan Endo-1
3-beta-Xylosidase
Xylosidases
Recombinant DNA
Genes
Fungal
Molecular Sequence Data
Microbiology
Gene Expression Regulation
Enzymologic
Fungal Proteins
taka-amylase-a
Amino Acid Sequence
zinc binuclear cluster
Gene
VLAG
beta-xylosidase
Binding Sites
Base Sequence
molecular-cloning
Aspergillus niger
Promoter
biology.organism_classification
Molecular biology
AFSG Staff Departments (WUATV)
chemistry
Trans-Activators
Zinc Cluster
escherichia-coli
amino-acid
5' Untranslated Regions
Sequence Alignment
Gene Deletion
DNA
Zdroj: Fungal Genetics and Biology, 35(2), 157-169
Fungal Genetics and Biology 35 (2002) 2
ISSN: 1087-1845
DOI: 10.1006/fgbi.2001.1321
Popis: By deletion across the promoter region of the xynF1 gene encoding the major Aspergillus oryzae xylanase, a 53-bp DNA fragment containing the XlnR binding sequence GGCTAAA as well as two similar sequences was shown to confer xylan inducibility on the gene. Complementary and genomic DNAs encoding the Aspergillus niger xlnR homologous gene, abbreviated AoxlnR, were cloned from A. oryzae and sequenced. AoXlnR comprised 971 amino acids with a zinc binuclear cluster domain at the N-terminal region and revealed 77.5% identity to the A. niger XlnR. Recombinant AoXlnR protein encompassing the zinc cluster region of the N-terminal part bound to both the consensus binding sequence and its cognate sequence, GGCTGA, with an approximately 10 times lower affinity. GGCTA/GA is more appropriate as the XlnR consensus binding sequence. Both sequences functioned independently in vivo in XlnR-mediating induction of the xynF1 gene. This was further confirmed by using an AoxlnR disruptant. Neither the xynF1 nor the xylA gene was expressed in the disruptant, suggesting that the xylan-inducible genes in A. oryzae may also be controlled in the same manner as described for A. niger.
Databáze: OpenAIRE
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