Characteristics of oseltamivir-resistant influenza A (H1N1) pdm09 virus during the 2013–2014 influenza season in Mainland China
| Autor: | Hejiang Wei, Weijuan Huang, Zhao Wang, Yanhui Cheng, Xiang Zhao, Minju Tan, Ning Xiao, Xiyan Li, Dayan Wang, Yuelong Shu, Yu Lan, Junfeng Guo |
|---|---|
| Jazyk: | angličtina |
| Předmět: |
Adult
Male China Oseltamivir Adolescent Medication history Sequence analysis viruses Molecular Sequence Data Mutation Missense Neuraminidase Hemagglutinin (influenza) Hemagglutinin Glycoproteins Influenza Virus Microbial Sensitivity Tests medicine.disease_cause Antiviral Agents Virus Microbiology Influenza A (H1N1) pdm09 Viral Proteins Young Adult chemistry.chemical_compound Influenza A Virus H1N1 Subtype Virology Drug Resistance Viral Influenza Human Influenza A virus medicine Humans Child Aged 50 % Inhibitory concentration biology Research Infant virus diseases Sequence Analysis DNA Middle Aged Infectious Diseases Amino Acid Substitution chemistry Antiviral-resistant Child Preschool Human mortality from H5N1 biology.protein Female |
| Zdroj: | Virology Journal |
| ISSN: | 1743-422X |
| DOI: | 10.1186/s12985-015-0317-1 |
| Popis: | Background In this study, we analyzed the characteristics of oseltamivir-resistant influenza A (H1N1) pdm09 virus isolated from patients in mainland China during the influenza season from September 2013 through March 2014, and provide guidance on which antiviral to be used for clinical treatment. Methods The all viruses collected from September 1, 2013 through March 31, 2014 were obtained from the Chinese National Influenza Surveillance Network. A fluorescence-based assay was used to detect virus sensitivity to neuraminidase inhibitors (NAIs). The hemagglutinin (HA) and neuraminidase (NA) gene of the oseltamivir-resistant viruses were sequenced. Results A total of 24 (2.14 %) influenza A (H1N1) pdm09 viruses that were resistant to oseltamivir were identified. These 24 viruses were isolated from 23 patients and no epidemiological link among them could be identified. Except for one virus with the H275H/Y mixture substitution, all the other 23 viruses had H275Y substitution in the NA protein. Sequence analysis revealed that the amino acid substitutions in the HA protein of influenza A (H1N1) pdm09 viruses with H275Y substitution isolated from mainland China were similar to the viruses from clustered cases reported in the United States, and the amino acid substitutions in the NA protein were similar to the viruses reported in Sapporo, Japan in 2013–2014. All of the oseltamivir-resistant viruses in mainland China and Japan possessed additional substitutions N386K, V241I and N369K in the NA protein, while most (>89 %) resistant-viruses from the United States during the same period possess V241I and N369K and did not have the N386K substitution. The N386K substitution was also exist in most sensitive viruses during the same period in mainland China. The amino acid substitutions in both HA and NA protein differed from the clustered cases from Australia reported in 2011 with additional substitutions. The drug-resistant influenza A(H1N1) pdm09 viruses were from patients without any known NAIs medication history prior to sampling. Conclusions During the influenza season from September 2013 through March 2014 in Mainland China, oseltamivir-resistant influenza A(H1N1)pdm09 viruses were much more frequently detected than ever since the appearance of the virus in 2009. Electronic supplementary material The online version of this article (doi:10.1186/s12985-015-0317-1) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
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