Limited Efficacy of Adipose Stromal Cell Secretome-Loaded Skin-Derived Hydrogels to Augment Skin Flap Regeneration in Rats
| Autor: | Vriend, Linda, van Dongen, Joris, Sinkunas, Viktor, Brouwer, Linda, Buikema, Henk, Moreira, Luiz, Gemperli, Rolf, Bongiovanni, Laura, de Bruin, Alain, van der Lei, Berend, Camargo, Cristina, Harmsen, Martin C, dPB RMSC, Dep Biomolecular Health Sciences, Pathobiologie |
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| Přispěvatelé: | dPB RMSC, Dep Biomolecular Health Sciences, Pathobiologie, Restoring Organ Function by Means of Regenerative Medicine (REGENERATE) |
| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: |
EXPRESSION
Swine wound healing ASC ASC secretome Humans Animals Rats Wistar hydrogels Secretome FIBROBLAST-GROWTH-FACTOR ECM Cell Biology Hematology skin flap VIABILITY Pepsin A Rats DIFFERENTIATION Adipose Tissue TISSUE FAT Culture Media Conditioned Delayed-Action Preparations MORPHOGENESIS SURVIVAL adipose-derived stromal cells Stromal Cells INJECTION Developmental Biology |
| Zdroj: | Stem Cells and Development, 31(19-20), 630. Mary Ann Liebert inc Stem cells and development, 31(19-20), 630-640. MARY ANN LIEBERT, INC |
| ISSN: | 1547-3287 |
| Popis: | Insufficient vascularization is a recurring cause of impaired pedicled skin flap healing. The administration of adipose tissue-derived stromal cells' (ASCs') secretome is a novel approach to augment vascularization. Yet, the secretome comprised of soluble factors that require a sustained-release vehicle to increase residence time. We hypothesized that administration of a hydrogel derived from decellularized extracellular matrix (ECM) of porcine skin with bound trophic factors from ASCs enhances skin flap viability and wound repair in a rat model. Porcine skin was decellularized and pepsin-digested to form a hydrogel at 37°C. Conditioned medium (CMe) of human ASC was collected, concentrated 20-fold, and mixed with the hydrogel. Sixty Wistar rats were included. A dorsal skin flap (caudal based) of 3 × 10 cm was elevated for topical application of DMEM (group I), a prehydrogel with or without ASC CMe (groups II and III), or ASC CMe (group IV). After 7, 14, and 28 days, perfusion was measured, and skin flaps were harvested for wound healing assessment and immunohistochemical analysis. Decellularized skin ECM hydrogel contained negligible amounts of DNA (11.6 ± 0.6 ng/mg), was noncytotoxic and well tolerated by rats. Irrespective of ASC secretome, ECM hydrogel application resulted macroscopically and microscopically in similar dermal wound healing in terms of proliferation, immune response, and matrix remodeling as the control group. However, ASC CMe alone increased vessel density after 7 days. Porcine skin-derived ECM hydrogels loaded with ASC secretome are noncytotoxic but demand optimization to significantly augment wound healing of skin flaps. |
| Databáze: | OpenAIRE |
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