Multiple-locus variable number of tandem repeat analysis (MLVA) of Irish verocytotoxigenic Escherichia coliO157 from feedlot cattle: uncovering strain dissemination routes
| Autor: | Séamus Fanning, Donal Minihan, Mary Murphy, Micheál O'Mahony, Paul Whyte, James F. Buckley |
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| Rok vydání: | 2008 |
| Předmět: |
DNA
Bacterial Cattle Diseases Multiple Loci VNTR Analysis Biology Escherichia coli O157 medicine.disease_cause Polymerase Chain Reaction law.invention Feces Tandem repeat law Genetic variation medicine Animals Escherichia coli Escherichia coli Infections Phylogeny Soil Microbiology Polymerase chain reaction DNA Primers Genetics lcsh:Veterinary medicine General Veterinary Strain (biology) Genetic Variation General Medicine bacterial infections and mycoses Housing Animal veterinary(all) Tandem Repeat Sequences lcsh:SF600-1100 Cattle Water Microbiology Variable number Ireland Research Article |
| Zdroj: | BMC Veterinary Research, Vol 4, Iss 1, p 2 (2008) BMC Veterinary Research |
| ISSN: | 1746-6148 |
| DOI: | 10.1186/1746-6148-4-2 |
| Popis: | Background The identification of the routes of dissemination of Escherichia coli (E. coli) O157 through a cohort of cattle is a critical step to control this pathogen at farm level. The aim of this study was to identify potential routes of dissemination of E. coli O157 using Multiple-Locus Variable number of tandem repeat Analysis (MLVA). Results Thirty-eight environmental and sixteen cattle faecal isolates, which were detected in four adjacent pens over a four-month period were sub-typed. MLVA could separate these isolates into broadly defined clusters consisting of twelve MLVA types. Strain diversity was observed within pens, individual cattle and the environment. Conclusion Application of MLVA is a broadly useful and convenient tool when applied to uncover the dissemination of E. coli O157 in the environment and in supporting improved on-farm management of this important pathogen. These data identified diverse strain types based on amplification of VNTR markers in each case. |
| Databáze: | OpenAIRE |
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