Effect of seminal plasma on dendritic cell differentiation in vitro depends on the serum source in the culture medium
| Autor: | C. van der Keur, Michael Eikmans, L. van der Westerlaken, Sebastiaan Heidt, F.H.J. Claas, Jacqueline D.H. Anholts, Johanna M. Kapsenberg, C. van Kooten, M.H.C. Craenmehr |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Male medicine.medical_treatment CD14 Fetal bovine serum Immunology Primary Cell Culture Cell Culture Techniques Serum Albumin Human chemical and pharmacologic phenomena Dendritic cell differentiation Peripheral blood mononuclear cell 03 medical and health sciences 0302 clinical medicine Immune system Semen medicine Immune Tolerance Immunology and Allergy Humans Fetal calf serum Cells Cultured Seminal plasma 030219 obstetrics & reproductive medicine Chemistry Human serum Obstetrics and Gynecology Cell Differentiation Serum Albumin Bovine hemic and immune systems Dendritic cell Dendritic Cells Molecular biology In vitro Culture Media 030104 developmental biology Cytokine Reproductive Medicine Leukocytes Mononuclear |
| Zdroj: | Journal of Reproductive Immunology, 137. ELSEVIER IRELAND LTD |
| Popis: | Dendritic cells (DCs) are key in shaping immune responses and are recruited to the human cervix after coitus by seminal plasma (SP). SP has been shown to skew the differentiation of monocyte-derived DCs towards an anti-inflammatory profile when cultured in medium containing fetal calf serum (FCS). Here, we confirmed that SP skewed DCs cultured in fetal bovine serum (FBS) towards a tolerogenic profile. To create a setting more similar to the in vivo situations in humans, we tested the immune regulatory effect of SP on DCs in cell cultures containing human serum (HS). SP-DCs cultured in HS did show increased CD14 and decreased CD1a, indicating an inhibited maturation phenotype. Gene expression of TGF-beta and IL-10 and IL-10 protein expression were elevated in LPS-activated SP-DCs, whereas IL-12p70 protein levels were decreased compared to LPS-activated control DCs. In contrast to FBS culture conditions, in the presence of HS co-cultures of SP-DCs with allogeneic peripheral blood mononuclear cells (PBMCs) did not result in decreased T cell proliferation and inflammatory cytokine production. Thus, under HS culture conditions SP can skew the differentiation of monocyte-derived DCs phenotypically towards alternatively activated DCs, but this immune regulatory phenotype is functionally less pronounced compared to SP-treated DCs cultured in FBS containing medium. These findings highlight the importance of the source of the serum that is used in SP treated cell cultures in vitro. |
| Databáze: | OpenAIRE |
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