A synthetic non-histone substrate provides insight into substrate targeting by the Gcn5 HAT and sirtuin HDACs
| Autor: | Michael Downey, Anthony Rössl, Mong-Shang Lin |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
chemistry.chemical_classification
0303 health sciences biology Chemistry Computational biology Substrate (biology) 03 medical and health sciences Synthetic biology enzymes and coenzymes (carbohydrates) 0302 clinical medicine Histone Enzyme Histone tails Gene expression Sirtuin biology.protein 030217 neurology & neurosurgery 030304 developmental biology A determinant |
| DOI: | 10.1101/345637 |
| Popis: | Gcn5 and sirtuins are highly conserved HAT and HDAC enzymes that were first characterised as regulators of gene expression. Although histone tails are important substrates of these enzymes, these proteins also target many non-histone substrates that participate in diverse biological processes. The mechanisms used by these enzymes to choose their non-histone substrates is unclear. In this work, we use a unique synthetic biology approach in S. cerevisiae to demonstrate that a shared target sequence can act as a determinant of substrate selection for Gcn5 and sirtuins. We also exploit this system to define specific subunits of the Gcn5-containing ADA complex as regulators of non-histone acetylations proteome-wide. |
| Databáze: | OpenAIRE |
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