Comparison between the effect of human Wharton’s jelly–derived mesenchymal stem cells and levetiracetam on brain infarcts in rats

Autor: Dalia M. Abd El Motteleb, Hala Mosaad, Samia Hussein, Mai M. Hasan
Rok vydání: 2018
Předmět:
Brain Infarction
Male
0301 basic medicine
Levetiracetam
Ischemia
Gene Expression
Phosphatidylethanolamine Binding Protein
Pharmacology
Mesenchymal Stem Cell Transplantation
Biochemistry
Neuroprotection
Brain ischemia
03 medical and health sciences
0302 clinical medicine
Memory
Neurotrophic factors
Glial Fibrillary Acidic Protein
medicine
Glial cell line-derived neurotrophic factor
Animals
Humans
Learning
Glial Cell Line-Derived Neurotrophic Factor
Wharton Jelly
education
Molecular Biology
education.field_of_study
biology
Caspase 3
business.industry
Brain-Derived Neurotrophic Factor
Mesenchymal stem cell
Cell Biology
medicine.disease
Rats
Neuroprotective Agents
030104 developmental biology
Phosphatidylethanolamine binding protein 1
biology.protein
business
030217 neurology & neurosurgery
Neurotrophin
Zdroj: Journal of Cellular Biochemistry. 119:9790-9800
ISSN: 1097-4644
0730-2312
DOI: 10.1002/jcb.27297
Popis: Background Stroke represents one of the major causes of death worldwide. Neuroprotection remains an important goal of stroke therapy. Stem cell therapeutic effect is attributed to the neuroprotective effect and the regulation of the oxidant stress. Levetiracetam (LEV), a second-generation antiepileptic drug, was reported to confer neuronal protection after cerebral ischemia reperfusion. Aim To investigate the effect of human Wharton's jelly-derived mesenchymal stem cells (WJ-MSCs) and LEV on the size of brain infarcts, the histological structure, the neurotrophic, and the antioxidant gene expression in middle cerebral artery occlusion in rats. Method The rats were divided into five equal groups of 12 rats each as follows. Sham control group: received phosphate-buffered saline (PBS); ischemia/reperfusion (I/R) group: received PBS before ligation; stem cell-treated group: the animal received MSCs before ligation; LEV-treated group: the animal received LEV before occlusion; combined group: the animals received both MSCs and LEV before occlusion. Hematoxylin and eosin staining was performed to study the histological structure of the brain. Real-time polymerase chain reaction (RT-PCR) was performed to assess gene expression. Results Both MSCs and LEV improved memory and learning in the treated groups compared with I/R group. Significant reduction of the infarct size in WJ-MSC- or LEV-treated groups when compared with untreated ones was found. By RT-PCR, a significant decrease of the expression values of glial-derived neurotrophic factor (GDNF), brain-derived neurotrophic factor (BDNF), phosphatidylethanolamine binding protein 1 (PEBP1), and copper-zinc SOD (Cu/ZnSOD) genes and a significant increase of pro-oxidant iNOS gene in the I/R rats compared with the sham group was detected. There was a significant increase in the expression values of GDNF, BDNF, PEBP1, and Cu/ZnSOD genes in both treated groups when compared with the I/R group. Rats treated with WJ-MSCs showed better results than rats treated with LEV. Finally, the combined use of LEV and WJ-MSCs was the most effective regimen as regard infarction volume and functional learning and memory tests. Conclusion In the brain ischemia model, combined WJ-MSCs and LEV have demonstrated striking protective effects in brain infarction by the modulation of the oxidant status and neuroprotective effect.
Databáze: OpenAIRE
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