NMR structural analysis of the capsular polysaccharide from Streptococcus pneumoniae serotype 6C
| Autor: | Mark Edward Ruppen, Ping Cai, Viliam Pavliak, Justin Keith Moran, Nancy Khoury, Olivier Marcq, Chenghua Deng |
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| Rok vydání: | 2012 |
| Předmět: |
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Magnetic Resonance Spectroscopy Streptococcus pneumoniae serotype Stereochemistry Molecular Sequence Data Capsules medicine.disease_cause Polysaccharide complex mixtures Biochemistry Analytical Chemistry stomatognathic system Streptococcus pneumoniae medicine Repeat unit chemistry.chemical_classification Chemistry Monosaccharides Polysaccharides Bacterial Organic Chemistry Stereoisomerism General Medicine Nuclear magnetic resonance spectroscopy Carbon-13 NMR carbohydrates (lipids) stomatognathic diseases Carbohydrate Sequence Two-dimensional nuclear magnetic resonance spectroscopy Heteronuclear single quantum coherence spectroscopy |
| Zdroj: | Carbohydrate Research. 351:98-107 |
| ISSN: | 0008-6215 |
| Popis: | The original structure of Streptococcus pneumoniae capsular polysaccharide (CPS) serotype 6C was proposed based on chemical degradation and tandem mass analysis [J. Clin. Microbiol.2007, 45, 1225-1233]. In order to confirm the repeat unit structure and assign the stereochemical structure, the CPS 6C and the known CPS 6A were fully characterized by NMR spectroscopy. Full (1)H and (13)C NMR spectra assignments of CPS 6C and CPS 6A were achieved based on DQCOSY, TOCSY, HSQC, HMBC, and NOESY analysis. These analyses confirmed the published structure of CPS 6A and established the repeat unit structure of the CPS 6C as: →2)-α-D-Glcp-(1→3)-α-D-Glcp-(1→3)-α-L-Rhap-(1→3)-D-Ribitol-(5→phosphate-. |
| Databáze: | OpenAIRE |
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