A Novel Surface-Exposed Polypeptide Is Successfully Employed as a Target for Developing a Prototype One-Step Immunochromatographic Strip for Specific and Sensitive Direct Detection of Staphylococcus aureus Causing Neonatal Sepsis
| Autor: | Aliaa A. Ali, Amani El-Kholy, Omneya M. Helmy, Noha M. Elhosseiny, Ahmed S. Attia, Sally A. Mohamed, Tamer M. Samir |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Peptide Biosynthesis Staphylococcus aureus cell wall protein neonatal sepsis Klebsiella pneumoniae 030106 microbiology Sample processing lcsh:QR1-502 Metal Nanoparticles medicine.disease_cause Biochemistry Sensitivity and Specificity lcsh:Microbiology Article one–step Chromatography Affinity Microbiology 03 medical and health sciences medicine Animals Humans Computer Simulation immunochromatographic strip Molecular Biology Specific immunoglobulins Antigens Bacterial Mice Inbred BALB C biology Neonatal sepsis Chemistry Biofilm Infant Newborn Computational Biology bioinformatics Staphylococcal Infections medicine.disease biology.organism_classification rapid 030104 developmental biology biology.protein direct detection Female Antibody Surface protein Peptides |
| Zdroj: | Biomolecules Biomolecules, Vol 10, Iss 1580, p 1580 (2020) Volume 10 Issue 11 |
| ISSN: | 2218-273X |
| Popis: | Neonatal sepsis is a life-threatening condition and Staphylococcus aureus is one of its major causes. However, to date, no rapid and sensitive diagnostic tool has been developed for its direct detection. Bioinformatics analyses identified a surface-exposed 112-amino acid polypeptide of the cell wall protein NWMN_1649, a surface protein involved in cell aggregation and biofilm formation, as being a species-specific and highly conserved moiety. The polypeptide was cloned, purified, and used to immunize mice to raise specific immunoglobulins. The purified antibodies were conjugated to gold nano-particles and used to assemble an immunochromatographic strip (ICS). The developed prototype ICS detected as low as 5 µ g purified polypeptide and 102 CFU/mL S. aureus within 15 min. The strip showed superior ability to directly detect S. aureus in neonatal sepsis blood specimens without prior sample processing. Moreover, it showed no cross-reaction in specimens infected with two other major causes of neonatal sepsis coagulase-negative staphylococci and Klebsiella pneumoniae. The selected NWMN_1649-derived polypeptide demonstrates success as a promising biomolecule upon which a prototype ICS has been developed. This ICS provides a rapid, direct, sensitive, and specific option for the detection of S. aureus causing neonatal sepsis. Such a tool is urgently needed especially in resources-limited countries. |
| Databáze: | OpenAIRE |
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