Hedgehog signaling and cell cycle control in differentiating erythroid progenitors
| Autor: | Anna N. Walker, William Gaffield, Kristina Detmer, R E Garner, Angela J. Thompson, Hassan Dannawi |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Cyclopamine
Biology Flow cytometry Hemoglobins chemistry.chemical_compound hemic and lymphatic diseases medicine Humans Hedgehog Proteins Glycophorins Propidium iodide Molecular Biology Hedgehog Cells Cultured Erythroid Precursor Cells medicine.diagnostic_test Cell Cycle Veratrum Alkaloids Cell Differentiation Cell Biology Hematology Cell cycle Hedgehog signaling pathway Erythropoietin receptor Cell biology Haematopoiesis chemistry Immunology Trans-Activators Molecular Medicine Biomarkers Signal Transduction |
| Zdroj: | Blood Cells, Molecules, and Diseases. 34:60-70 |
| ISSN: | 1079-9796 |
| Popis: | Hedgehog (Hh) signaling regulates differentiation in numerous systems, but its functions in the control of hematopoietic differentiation have not been extensively explored. Initial studies have indicated that hedgehog signaling affects the proliferation and differentiation of erythroid progenitors (Detmer, K., et al., Erythroid differentiation in vitro is blocked by cyclopamine, an inhibitor of hedgehog signaling. Blood Cells Mol. Dis. 26(4) (2000) 360-372). To examine the effect of Hh signaling on the erythroid developmental program at the molecular level, Hh signaling in committed erythroid progenitors differentiating in vitro was inhibited, and the appearance/disappearance of molecular markers of erythroid differentiation was monitored. The expression timetable for CD34, CD36, the erythropoietin receptor, and glycophorin A was retarded in the absence of Hh signaling. Hemoglobinization was delayed and decreased relative to controls. Morphological changes of erythroid maturation were also delayed. The fraction of cells in S-phase was decreased during the initial period of exponential expansion as assessed by propidium iodide staining and flow cytometry, as was the rate of tritiated thymidine incorporation. A modest decrease in the proliferation rate was observed. These results suggest that Hh signaling is one of the mechanisms in the regulation of erythroid proliferation and differentiation. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect