Tumstatin attenuates the promotion effect of IL-17 secreted by Th17 cells on the stemness maintenance of glioma cells
Autor: | Yigao Lu, Wei Shen, Wei Yu, Haiwei Le, Weihua Xu, Wangfang Yu, Jun’an Hu |
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Rok vydání: | 2021 |
Předmět: |
0301 basic medicine
Adult Collagen Type IV Male Tumstatin Cell Mice Nude Antineoplastic Agents Biology Peripheral blood mononuclear cell Autoantigens Pathology and Forensic Medicine 03 medical and health sciences 0302 clinical medicine Cell Movement Glioma Cell Line Tumor medicine Tumor Microenvironment Animals Humans Neoplasm Invasiveness Cell Proliferation Tumor microenvironment Mice Inbred BALB C Brain Neoplasms Interleukin-17 Cell Biology Transfection Middle Aged medicine.disease Xenograft Model Antitumor Assays Tumor Burden 030104 developmental biology medicine.anatomical_structure 030220 oncology & carcinogenesis Case-Control Studies Cancer research Neoplastic Stem Cells Th17 Cells Female Interleukin 17 Stem cell Transcription Factors |
Zdroj: | Pathology, research and practice. 223 |
ISSN: | 1618-0631 |
Popis: | The presence and clinical significance of IL-17 and IL-17-expressing cells have been studied for several cancers, although their correlation with tumor development remains controversial. Peripheral blood was collected from healthy donors and glioma patients to isolate peripheral blood mononuclear cells (PBMCs). The percentage of IL-17-expressing cells and the production of inflammatory cytokines in PBMCs and tissues were measured. Human IL‑17 cDNA was then inserted into the pEGFP‑N1 plasmid and transfected into the glioma U87MG cell line, and tumstatin was used to block the effect of the IL-17 overexpression. Stem cell transcription factors were evaluated in each group using qRT-PCR and western blotting, and proliferation and migration were detected using colony formation and wound-healing assays. The cells were then subcutaneously inoculated into nude mice to evaluate the growth of glioma. Compared with healthy donors, the PBMCs from glioma patients showed a significant accumulation of IL-17-expressing T cells. Th17 cell differentiation-related cytokines (IL-23, TGF-β and IL-6) were increased in the tumor microenvironment. IL‑17 transfection increased the mRNA and protein expression of stem cell transcription factors in U87MG cells in vitro. The proliferation and migration of U87MG cells were also increased. Moreover, the pEGFP‑N1‑IL‑17‑U87MG cells grew more rapidly than other cells. However, tumstatin-treated U87MG cells showed significantly inhibited the effects of IL-17 overexpression. Tumstatin effectively suppressed IL-17-derived U87MG cell growth by downregulating stem cell maintenance factors and inducing proliferation and migration. These findings indicated that IL-17 represents a potential prognostic marker for glioma, while tumstatin has potential in the treatment for glioma. |
Databáze: | OpenAIRE |
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