A Danish cholinesterase research unit
| Autor: | H. K. Hanel, J. Viby-Mogensen |
|---|---|
| Rok vydání: | 1977 |
| Předmět: |
Male
medicine.medical_specialty Succinyldicholine Genotype Apnea Denmark Dibucaine Succinylcholine Danish chemistry.chemical_compound Internal medicine Sodium fluoride medicine SUXAMETHONIUM SENSITIVITY Cholinesterases Humans In patient Cholinesterase Serum cholinesterase biology business.industry Research Genetic Variation General Medicine language.human_language Surgery Pedigree Anesthesiology and Pain Medicine chemistry language biology.protein Female business medicine.drug |
| Zdroj: | Acta anaesthesiologica Scandinavica. 21(5) |
| ISSN: | 0001-5172 |
| Popis: | The organisation and initial experiences of a Danish Cholinesterase Research Unit are presented. The primary aim of the research unit is to provide a service for determining genotypes and activity of serum cholinesterase (acylcholine-acyl-hydrolase E.C. 3.1.1.8.) in patients suspected of having abnormal serum cholinesterase. Centralization of these investigations and use of a standard methodology have permitted the setting up of a central card index of families in Denmark with inherited abnormal serum cholinesterase. During the first 3 years, a total of 967 individuals have been genotyped. Two hundred patients were referred to the centre following an episode of prolonged apnoea after suxamethonium. In addition, 723 relatives of these 200 patients have been investigated, and 513 warning cards have been issued. Forty-four individuals were genotyped for reasons not related to prolonged apnoea. Genetic variations of serum cholinesterase were identified with differential inhibition techniques using dibucaine, sodium fluoride, sodium chloride, succinyldicholine, and urea as inhibitors. It was found that no single inhibitor was able to differentiate between all known genotypes of locus E1. Inhibition of serum cholinesterase by urea, used together with the conventional dibucaine inhibition, gave results which compared favourably with the results obtained with the classical combination of dibucaine and fluoride inhibition. The results indicated that, at least in differentiation of rarer genotypes, urea inhibition may give valuable information not obtainable using fluoride inhibition. The difficulties in deciding which patients should be issued with warning cards are discussed. It is pointed out that only through close cooperation with the local anaesthetists involved will it be possible to elucidate the connection between the rarer genotypes and suxamethonium sensitivity. |
| Databáze: | OpenAIRE |
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