ABI-like transcription factor gene TaABL1 from wheat improves multiple abiotic stress tolerances in transgenic plants
| Autor: | Ming Chen, Yao-feng Chen, Shiqing Gao, Xueyin Li, Zhao-Shi Xu, You-Zhi Ma, Lian-Cheng Li, Yi-Miao Tang, Dong-Bei Xu, Chang-Ping Zhao |
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| Rok vydání: | 2014 |
| Předmět: |
Transgene
Arabidopsis Gene Dosage Genetically modified crops Biology Genes Plant chemistry.chemical_compound Gene Expression Regulation Plant Stress Physiological Freezing Tobacco Botany Genetics Gene Abscisic acid Transcription factor Triticum Plant Proteins Regulation of gene expression Abiotic stress Protoplasts fungi food and beverages Salt Tolerance General Medicine Plants Genetically Modified Adaptation Physiological Cell biology Oxidative Stress Protein Transport chemistry Plant Stomata Mesophyll Cells Transcription Factor Gene Abscisic Acid Subcellular Fractions Transcription Factors |
| Zdroj: | Functional & Integrative Genomics. 14:717-730 |
| ISSN: | 1438-7948 1438-793X |
| DOI: | 10.1007/s10142-014-0394-z |
| Popis: | The phytohormone abscisic acid (ABA) plays crucial roles in adaptive responses of plants to abiotic stresses. ABA-responsive element binding proteins (AREBs) are basic leucine zipper transcription factors that regulate the expression of downstream genes containing ABA-responsive elements (ABREs) in promoter regions. A novel ABI-like (ABA-insensitive) transcription factor gene, named TaABL1, containing a conserved basic leucine zipper (bZIP) domain was cloned from wheat. Southern blotting showed that three copies were present in the wheat genome. Phylogenetic analyses indicated that TaABL1 belonged to the AREB subfamily of the bZIP transcription factor family and was most closely related to ZmABI5 in maize and OsAREB2 in rice. Expression of TaABL1 was highly induced in wheat roots, stems, and leaves by ABA, drought, high salt, and low temperature stresses. TaABL1 was localized inside the nuclei of transformed wheat mesophyll protoplast. Overexpression of TaABL1 enhanced responses of transgenic plants to ABA and hastened stomatal closure under stress, thereby improving tolerance to multiple abiotic stresses. Furthermore, overexpression of TaABL1 upregulated or downregulated the expression of some stress-related genes controlling stomatal closure in transgenic plants under ABA and drought stress conditions, suggesting that TaABL1 might be a valuable genetic resource for transgenic molecular breeding. |
| Databáze: | OpenAIRE |
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