Co-occurrence of mcr-1, mcr-3, mcr-7 and clinically relevant antimicrobial resistance genes in environmental and fecal samples
| Autor: | João Pedro Rueda Furlan, Eliana Guedes Stehling, Lucas David Rodrigues dos Santos, Lucas Victor Pereira de Freitas, Inara Fernanda Lage Gallo, Micaela Santana Ramos |
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| Rok vydání: | 2020 |
| Předmět: |
Aerobic bacteria
Polymerase Chain Reaction Biochemistry Microbiology MICROBIOLOGIA DA ÁGUA Feces 03 medical and health sciences Plasmid Drug Resistance Multiple Bacterial Drug Resistance Bacterial Genetics Animals Replicon Typing Molecular Biology Gene Soil Microbiology 030304 developmental biology 0303 health sciences Bacteria biology 030306 microbiology General Medicine Amplicon biology.organism_classification Animals Zoo MCR-1 Water Microbiology hormones hormone substitutes and hormone antagonists Plasmids |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| Popis: | Multidrug-resistant bacteria harboring different antimicrobial resistance genes (ARGs) have been detected worldwide. The association of plasmid-mediated colistin resistance genes (mcr-like) and other ARGs in bacteria isolated from animals is a huge concern worldwide. Therefore, this study aimed to investigate the presence of mcr-like genes and clinically relevant ARGs as well as plasmids in samples from a zoo. Fecal and environmental (soil and water) samples were collected from a zoo and the DNA of cultivable aerobic bacteria was extracted. ARGs were screened by PCR and the plasmids were detected using the PCR-based replicon typing method. A total of 74 amplicons from 27 ARGs [mcr-1, mcr-3, mcr-7.1, blaCTX-M-Gp1, blaCTX-M-Gp2, blaCTX-M-Gp9, blaVEB, blaPER, blaCMY, tetA, tetB, tetC, aadA, aac(6')-Ib, aph(3')-Ia, ant(2'')-Ia, qnrA, qnrB, qnrS, oqxA, oqxB, sul1, sul2, sul3, cmlA, mefAE, ermB] and 21 amplicons from eight plasmid families (IncY, ColE-like, IncFrepB, IncFIA, IncFIB, IncHI1, IncFIC, IncP) were detected. These findings reinforce that the zoo acts as a reservoir of clinically relevant ARGs, including mcr-like, and call attention to the monitoring studies in the zoo. Therefore, to the best of our knowledge, this is the first report of the world of mcr-1, mcr-3 and mcr-7.1 in environmental samples from the zoo. |
| Databáze: | OpenAIRE |
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