Expression of Transferrin mRNA in Rat Oligodendrocytes is Iron-independent and Changes with Increasing Age
| Autor: | Phillip S. Oates, Torben Moos, Evan H. Morgan |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
0301 basic medicine
Aging medicine.medical_specialty Cell type P50 Transcription Genetic Iron Medicine (miscellaneous) Corpus Callosum 03 medical and health sciences 0302 clinical medicine Reference Values Transcription (biology) Internal medicine medicine Animals Inducer RNA Messenger chemistry.chemical_classification Messenger RNA 030109 nutrition & dietetics Nutrition and Dietetics General Neuroscience Transferrin RNA Iron Deficiencies General Medicine Rats Oligodendroglia Endocrinology Hematocrit chemistry Choroid plexus 030217 neurology & neurosurgery |
| Zdroj: | Europe PubMed Central |
| ISSN: | 1476-8305 1028-415X |
| DOI: | 10.1080/1028415x.2001.11747347 |
| Popis: | As transferrin in the brain may originate principally from synthesis by three different cell types, i.e. hepatocytes, oligodendrocytes and choroid plexus, this study employed a morphological analysis to specifically address oligodendrocytic expression of transferrin mRNA in young (P17) and adult (P50) rats. In spite of a lowering of the concentration of brain iron by approximately 22% in the young iron deficient rats transferrin mRNA expression in oligodendrocytes was not affected when measured by quantitative densitometry. In adult rats, the baseline transferrin mRNA expression in oligodendrocytes was higher than in the young animals, but did not change in spite of a reduction in brain iron by approximately 19%. Brain iron and transferrin mRNA expression in oligodendrocytes were unaltered in iron overloaded rats when compared to age-matched controls. As transferrin expression was lower in the young rat, when constituents from the blood have a relatively higher concentration in the brain than during adulthood, it seems unlikely that blood-borne factors such as transition metals act as inducers of transferrin gene expression in oligodendrocytes. Instead, the higher but constitutive expression of transferrin mRNA at later ages, when the blood-brain barrier segregates the brain from other body parts, may indicate that molecules released from the brain interior are responsible for regulating transcription of the transferrin gene. |
| Databáze: | OpenAIRE |
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