Impacts of protein supplementation during late gestation of beef cows on maternal skeletal muscle and liver tissues metabolism
| Autor: | P. D. Teixeira, A. S. Trece, Marcio de Souza Duarte, L. R. Santos, Mateus Pies Gionbelli, Thaís C Costa, R. C. Lopes, T.R.S. Gionbelli, Cláudia Batista Sampaio |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Male
040301 veterinary sciences Biology SF1-1100 0403 veterinary science chemistry.chemical_compound Animal science Pregnancy medicine Animals Muscle Skeletal tissue mobilization chemistry.chemical_classification amino acids Fetus Methionine 0402 animal and dairy science Fatty acid Skeletal muscle 04 agricultural and veterinary sciences Metabolism Animal Feed 040201 dairy & animal science Animal culture Diet Amino acid fetal programming gluconeogenesis medicine.anatomical_structure Liver chemistry Gluconeogenesis Dietary Supplements Gestation Cattle Female Animal Science and Zoology maternal nutrition |
| Zdroj: | Animal, Vol 14, Iss 9, Pp 1867-1875 (2020) |
| ISSN: | 1751-7311 |
| DOI: | 10.1017/s1751731120000336 |
| Popis: | Since nutritional requirements are increased at the end of gestation to meet the demands of the pregnant uterus, pregnant beef cows are susceptible to mobilization of body reserves (mainly fat and amino acids (AAs)) and to alter the metabolism of nutrients in the liver and muscle to support such demands. The objective of this study was to evaluate the effect of CP supplementation on maternal nutrient metabolism in the late gestation of beef cows grazing a low-quality pasture. Forty-three pregnant Nellore cows gestating male fetuses (average age = 6 years; average weight = 544 kg) at 193 ± 30 (mean ± SD) days (d) of gestation were divided into eight groups (experimental units, with four to five cows each). Treatments were (1) control (CON, n = 4): pasture-based (PB) diet without CP supplementation and (2) supplemented (SUP, n = 4): PB diet daily supplemented with 2 g/kg of BW of a 43.5% CP supplement. Liver and skeletal muscle biopsies were performed at 265 days of gestation and samples were collected for mRNA expression. On day 280 of gestation, blood samples were collected to assess plasma levels of AA. The CON-fed cows tended to have greater (P = 0.057) total circulating AA than SUP-fed cows. The circulating glycogenic AA was greater (P = 0.035) in CON than in SUP cows. CON cows was greater for histidine (P = 0.015), methionine (P = 0.007) and alanine (P = 0.036) than SUP cows. The CON- and SUP-fed showed no differences for gluconeogenesis, fatty acid transport and signaling axis markers in the liver. The mRNA expression of markers for skeletal muscle synthesis, p7056k (P = 0.060) and GSK3B (P = 0.096), tended to be greater in cows from CON than SUP group. No differences were found for mRNA expression of markers for skeletal muscle degradation. We conclude that CP supplementation to CP-restricted late-pregnant beef cows reduces the maternal tissue mobilization and changes the profile of plasma circulating AA and the mRNA expression of markers for the synthesis of skeletal muscle tissue. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|